ObjectiveCartilage defects is always a big problem in the clinical work. The limit source of autologous cartilage and the high absorption rates of allograft cartilage are limiting the application of adult cartilage tissue.Recently the development of tissue engineering has brought new solutions to this problem,a large number of studies on cartilage tissue engineering drive the [...]
Objective:To compare the chondrogenic potential of rabbit bone marrow-derived mesenchymal stem cells (BMSCs) and adipose tissue-derived stem cells (ADSCs) in vitro.Methods:ADSCs were isolated from the subcutaneous adipose tissue which was from 6-month-old New Zealand white rabbits’abdomen by mechanical digestion and enzyme digestion, and then cultured and amplified in vitro. BMSCs were obtained from femurs of [...]
Tissue engineering is an interdisciplinary field that applies the principles of engineering and life sciences toward the development of biological substitutes that restore, maintain, or improve tissue function or a whole organ. Scaffold material is one of the three factors of tissue engineering. Following the guidance of the performance standards of scaffold materials in tissue [...]
Objective:(1) To research the characteristics of rabbit corneal endothelial cells(CECs) which were cultured in vitro, and to lay a foundation for tissue engineering of corneal posterior lamellar. (2)To investigate the effects of EDTA on the proliferation of cultu-red rabbit cornea endothelial cell in vitro. (3) To investigate the biocompatibility of small intestinal submucosa (SIS) with [...]
The destruction and lost of articular cartilage and subchondral bone caused by inflammation, trauma and tumor often leads to dysfunction of articular. Besides, no matter the destruction origin from the articular cartilage or the subchondral, the defect will deep into subchondral bone at last. Previous researches mainly focus on cartilage tissue engineering, and pay less [...]
Objectives:Human bone mesenchymal stem cells (hBMSCs) have been widely used as seed cells in bone tissue engineering. The dose effect of hBMSCs on bone tissue engineering is important for effective application and has not been identified. In our study, we detect the osteogenic related gene expression during in vitro osteogenic differentiation of hBMSCs and characterize [...]
Objective:Periodontal ligament cell populations were isolated from the periodontal membrane of extracted teeth and were purified; On this basis, the purified cells were identified. This paper confirmed that the cells were “stem cells” cells, to provide a basis for follow-up study of periodontal tissue engineering experimental.Methods:1. Separation and purification of human periodontal ligament stem cells [...]
Objective: To find an efficient method to induce rabbit bone marrow mesenchymal stem cells different towards smooth muscle cells in vitro, so as to establish a stabile cells source for further study.Methods: Rabbit bone marrow mesenchymal stem cells were isolated from bone marrow by the method of density gradient centrifuge. By adhering culture, a further [...]
Objective:To study the survival and distribution of allogenic BMSCs after implantation into other rabbits. To evaluate osteogenetic effectiveness of gelatin sponge mixed with BMSCs of rabbit induced by LMP-1 in radial defects repair of rabbits, to lay the foundation for further application. Methods:The BMSCs of New Zealand white rabbits were collected,cultured,purified and amplified,then they were [...]
Objective The bone defect caused by trauma and bone disease is one of difficult problems of clinical work. Following the progress of DNA recombiant and transfer technology and tissure engineer technology, the treatment of bone defect obtained rapid development. LMP as a bone formation inducted protein was discovered recently, it’s proved that LMP-1 and 3 [...]
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