The core of tissue engineering is to build a complex three-dimensional space ofcells and porous materials, which is to promote the implantation of cell’s adhesion,proliferation, differentiation and metabolism. Therefore, how to effectively prepared tomeet the needs of the porous scaffold is of concern. Among many fabricated methods,solvent casting/porogen leaching (SC/PL) is one of the most [...]
BackgroundThe sternum is a flat bone located in the middle of the chest wall，The shape ofdagger，Sternal manubrium, sternum, xiphoid. Sternum on both sides of ribsconnected to constitute the thorax, since an important role in the protection of themediastinum and chest visceral. If the sternum lesions occur, such as: congenitalmalformations, dysplasia, refractory sternal osteomyelitis, traumatic sternalcomminuted, [...]
ObjectiveFor severe injury of ligaments, natural healing fails to take place and replacementtissues or grafts are required. Autografts tissues have been widely used in clinic. Butdonor site morbidity, prolonged surgery time, scar proliferation and sacrifice ofautogenous tissues remain to be the limiting factors of autografts. Tissue-engineeredligaments (TELs) have become the research focus of injuried ligaments [...]
Objective:1.To investigate the ability of Multilineage differentiation of human umbilicalcord-MSCs by subculturing to23passages.2. To investigate a better method of inducinghUC-MSCs into chondrocytes in non-scaffold culture in vitro.Methods: Mesenchymal stem cells were isolated from umbilical cord by collagenaseⅡdigestion and were digested by trypsin for continuous passage culture. A series of differentgeneration cultured-cells were collected for following [...]
ObjectiveTo investigate the feasibilities of using the containing nanofiber scaffolds, saltingprepared silk fibroin scaffolds which are constructed the lamina propria of the oral mucosain vitro, and to provide experimental evidence for tissue-engineered oral mucosa in thefuture.MethodsThe SD rats’ primary oral mucosal fibroblasts (oral fibroblast cell) were cultured bytissue adherent method. The third generation of oral [...]
The research on bone tissue engineering materials which are used for replacing damaged tissues has extensive implications since the bone tissue which is lost or damaged as a result of injury, infection and ageing becomes increasingly serious. Due to the self-assembly and biomineralization in the long term evolution, natural biomaterials become a kind of material [...]
Recently, tissue engineering, which applies methods from engineering and lifesciences to create arti cial constructs to direct tissue regeneration, has attracted manyscientists and surgeons with a hope to treat patients in a minimally invasive and lesspainful way. The core of tissue engineering: the complex three-dimensional space,namely the establishment of cells and biological materials with the [...]
Objective To explore the ability of using vascularized tissue engineered bone packed by the greater omentum to repair bone long bone defect, and assess the feasibility and quality of tissue engineering bone packed by the greater omentum to repair long bone defects. It will supply and supplement experimental basis for tissue engineering bone in clinical [...]
Objective：In this study, composites composed of organic polymer material polyvinylalcohol and natural polymer hyaluronic acid was modified by the addition of collagento prepare a PVA-HA-COL composite scaffold. Furthermore, we assessed thefeasibility of PVA-HA-COL composite used as a tissue engineering scaffold.Method：By way of film-forming and freeze-drying, different molecular weightpolyvinyl alcohol-hyaluronic acid composite scaffold was prepared by [...]
Objective:To study the effect of platelet lysate(PL) during the induction course from human umbilical cord derived mesenchymal stem cells(hUCMSCs) to chondrocytes in vitro.Methods:HUCMSCs were isolated by collagenase digestion. The surface markers of the cells were detected by flow cytometer. According to the different components of inductive medium we divided the study into the following three [...]
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