Background leukemia with AML1/ETO fusion gene positive is a subtype of acute myeloid leukemia, and it is characterized by chromosome translocation of t(8; 21)(q22; q22) which generates the fusion gene of AML1/ETO leading to leukemia through several channels. AML1/ETO fusion gene could activate JNK signal transduction pathway so as to up-regulate the expression of c-jun [...]
Backgroud Prostate adenocarcinoma is the most commonly diagnosed noncutaneous cancer and second leading cause of cancer related deaths in men. Death due to prostate cancer is attributed to metastatic disease in which primary tumors evade the prostate capsule and eventually metastasize. This presents a large problem considering that current diagnostic procedures do not readily determine [...]
[Objective and significance]Breast cancer is the most commonly found carcinoma of female breasts, and also one of the most common malignant tumor in women, whose incidence and mortality has been increasing in recent years, ranking the top of the female carcinoma. Currently, the common treatments for breast cancer are the traditional surgical treatment, supplemented by [...]
Objective:To investigate positive effect of CRMP1 gene on neurite outgrowth and cytoskeleton in rat hippocampal neurons.Materials and methods:The three-pair siRNAs acquired via chemical synthesis were screened by RT-PCR and real-time PCR. The effective siRNA interfering CRMP1 gene was supported by immunocytochemical and western blot. The fluorescence radix FAM was constructed upon 5′terminal of the siRNA, [...]
Objective:To observe the hepatocarcinogenic effect of HBV x, the most potential oncogenic factor, interference on cell growth and the expression of hTERT and MMP-2 genes in human hepatocellular carcinoma HepG2.2.15 cells, which constitutively produce HBV x. Methods:(1) Identification and amplification of HBV x gene expression vector pSIHBV/X. (2) The human hepatocellular carcinoma HepG2.2.15 cell culture. [...]
Objective Esophageal cancer is a common digestive system, accounting for 2% of all malignant tumors. Esophageal cancer is a high incidence in China, the death rate of which in the second, however, its mechanism remains unkonow. Tumor is a long-term multi-factor, multi-step complex process, including cell proliferation and differentiation, apoptosis, cell adhesion, circumventing the immune [...]
Objective:In our early studies, we have shown that proto-oncogenes c-myc and neul up-regulate during primordial follicle assembly and development using the microarray approach. In this experiment, we further investigated the role and the possible related-signaling pathway of proto-oncogenes c-myc and neul in the initiation of primordial follicle growth in rats by using RNA interference and [...]
Objective: To detect the application of DREAM silencing through siRNA mediated by lentiviral vector in gene therapy of rats neuropathic pain due to chronic constriction injury of the sciatic nerve (CCI), and to observe the changes of astrocyte activity and phosphorylation of cyclic AMP response element binding protein (pCREB) under the control of RNA interference(RNAi). [...]
Objective:To explore the effects of survivin small interference RNA (siRNA) on the cell differentiation and sensitivity of K562 cell line to Adriamycin (ADM), which will offer an important theory evidence for chemotherapy of leukemia.Method: 1.The expression of survivin in K562 cells after treated with recombinant plasmid pshRNA-survivin. Recombinant plasmid were delivered in the form of [...]
Leukemia is a heterogenicity disease, caused by clone proliferation of hematopoietic stem cell. The main treatment measure of leukemia is chemotherapy targeting leukemic cells, but there are some inevitable problem, such as relapse, metastasis, drug-resistance and so on. Targeting treatment of leukemia at molecular level is the present research hotspot.Neclephomin (NPM) is a multifunctional protein, [...]
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