Modification of PI3K Signaling Pathway under the Driving of KDR Promoter/Enhancer Influences the Proliferation and Apoptosis of Rat Vascular Endothelial CellObjective:The vascular endothelial cell is one of the important components of the vessel wall. Damage of VEC promotes the restenosis after coronary bypass grafting. The purpose of this study is to construct the KDR specific [...]
Objective: To design and select effective siRNA targeting human IGF-II and to construct thevector of recombinant RNA polymerase II hAFP/hTERT dual promoters-driven siRNAexpression against human IGF-II gene.Method:①According to the siRNA design principle, three siRNAs (siRNA1、siRNA2、siRNA3) targeting IGF-II mRNA and one negative control siRNA （NC siRNA）were designedand synthesized.25nM,50nM,100nM three different concentrations of siRNA1-3weretransfected into Huh7cells through [...]
Bronchial asthma is a chronic disease that occur at all ages and can seriously affect thequality of life，even threaten people’s health. Atopic dermatitis, allergic rhinitis, andbronchial asthma are allergic disorders characterized by a predominance of Th2polarisation,the synthesis of IgE, and mast cell and eosinophil-associated inflammation. The cytokineenvironment when the initial antigen stimulation determines the direction [...]
Objective The study on the relationship between the specific high expression of theBrachyury gene in chordoma cells with the apoptosis of chordoma cell. Using RNAinterference, we constructed the recombinants mediated by lentivirus vectorpLenti-Bra-shRNA to express si-Brachyury. After transfected these recombinants tochordoma cell line CHZP, we studied their resistance to Paclitaxel. We expected toexplore the possible [...]
Objective To study the influence of silencing Med19on metastasis and invasion ofhuman breast cancer MCF-7cells， further explored Med19gene role and activemechanism in metastasis and invasion of the breast cancer.Method Construction of lentivirus expression vector according to the sequence ofMed19gene, then transfection of human breast cancer MCF-7cell line withsiRNA-Med19lentirivus expression vector. RT-PCR and Western blot were [...]
Objective To approach the association between the mRNA expression ofASPP family genes and cisplatin chemotherapy sensitivity in gastric cancercells of different p53genetic background. To approach the influence ofcisplatin chemotherapy sensitivity to gastric cancer cells of different p53genetic background after the mRNA expression of iASPP weredownregulated.Methods （1）Gastric cancer cell lines AGS (wild-type p53) andSGC-7901(mutant-type p53) with [...]
Prostate cancer is one of the malignant tumors that occur in male prostate tissue, which is resulted from prostate gland bubble abnormal cellular sprawling growth. The risk of prostate cancer has obvious geographic and racial differences. In Europe, United States and other developed countries and regions, it is the most common cancer in men with [...]
ObjectiveTo observe role of targeting inhibition of prostate cancer antigen-1expression for prostate cancer xenografts in nude mice.MethodsWe inject human prostate cancer LNCaP cells into subcutaneous tissue of nude mice to establish prostate cancer xenografts in nude mice. The mice were randomly divided into experimental and control groups. Experimental group:the recombinant plasmid PSMAe/p-PCA-1-pA-Sliencer was injected into [...]
Breast cancer is one of the most common malignant tumors in women. Whetherin developed or developing countries, the incidence and mortality rates of breastcancer are relatively high. The causes of breast cancer are quite complex, in additionto genetic factors, high-fat diet, obesity, hormones, immune, and variousenvironmental factors (physical and chemical, biological factors and lifestyle, etc.)may [...]
Rotator cuff disease (RCD) is the most common cause of shoulder pain anddysfunction. Pathophysiology and biochemical mechanism of RCD have been identified.With the development of cell biology and molecular biology,we know Subacromial bursitiswas regarded as the major souce of pain and dysfunction in rotator cuff disease. By thenon-specific stimuli such as mechanical stretch, hypoxia, inflammatory [...]
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