Objective:1.To explore the serum protein mass spectrum of the cervical intraepithelial neoplasia (CIN) III, cervical carcinoma and healthy women to screen its potential special biomarker by surface-enhanced laser desorption/inionation-time of flight-mass spectrometry (SELDI-TOF-MS) and weak cation exchange (WCX) chip, and to evaluate its clinical significance.2. To screen and identificate differential expression proteins of Hela cells [...]
Objective:To compare and analyze the protein expression profiles of extensively drug-resistant (XDR) and multidrug-resistant(MDR)tuberculosis (TB) isolates, and identify differentially expressed protein spots related to XDR-TB, and to detect related genes expression changes in Mycobacterium tuberculosis XDR/MDR and the standard strain H37Rv.Methods:Total proteins extracted from clinical isolates of XDR-TB and MDR-TB as well as the standard [...]
Objective:Constructing rat ischemia/reperfusion injury(IRI) model to investigate the protective effect of ischemic postconditioning on ischemic rat myocardium which suffered IRI. Myocardial mitochondria was purified and the mitochondrial protein line 2D-DIGE comparative was performed,to explore the effect of protein expression in mitochondria following ischemic postconditioning.Methods:1 Sprague-Dawley rats were divided randomly into 4 groups(n=6, each group), i.e. [...]
Objective:Constructing rat ischemia/reperfusion injury (IRI) model to investigate the protective effect of diazoxide postconditioning on ischemic rat myocardium which suffered IRI, and to profile protein expression in mitochondria following the treatment of diazoxide.Methods:Langendorff apparatus were used to establish the model of myocardial ischemia reperfusion injury.24 male Sprague-Dawley rats were chosen randomly and divided into 4 [...]
Object:To establish chronic cerebral ischemia rat model and to explore the mechanisms of shenlongjiannao capsule which can inhance the learning and memory ability of chronic cerebral ischemia rats.Method:The chronic cerebral ischemia model rats were established by two-vessel occlusion.50 rats were randomly divided into 5 groups:sham operatio-n group,model group, shenlongjiannao capsule groups(high and low dose) a-nd [...]
Cadmium (Cd) is toxic to Leydig cells, which directly decreases testosterone secretion, but its mechanism is still poorly understood. To further explore the signal pathway of toxicity caused by Cd, different concentrations (IC25, IC50 and IC75) of Cd were exposed to R2C cells for 24 h; 2DE-based proteomics was used to analyze the altered expression [...]
AIM:Proteomics was used to screen the biomarkers for predicting skin irritation. The protein-protein interaction between differentially expressed proteins biomarkers was studied, which would be helpful for the mechanistic understanding of the skin irritation.Methods:Keratinocytes were exposed to representative acid/basic irritants for 4h, and continued to culture for another 0,2,4,8 and 16h in the normal culture medium, [...]
Objective:Skeletal muscle plays a major role in whole body protein metabolism, We hypothesized that relatively intense aerobic conditioning of eccentric exercise would increase the activity of metabolic enzymes associated with muscle oxidative capacity, and characterization of cytoskeleton proteins response to resistance exercise would come into being adaptation.Methods:Sprague-Dawley male healthy rats(220-250g) were divided randomly into four [...]
SUBJECT1. To establish two-dimensional electrophoresis profiles with high resolution and reproducibility from hypothalamuses and adrenal glands of normal rats、hyperensive rats with hyperactivity of liver-yang and the hypertensive rats with hyperactivity of liver-yang with Pinggan-Qianyang treatment.2. To explore the different expression in hypothalamic and adrener-gdrenic proteins of hypertensive rats with hyperactivity of liver-yang with Pinggan-Qianyang treatment, [...]
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