Objective:By adopting the method of cell culture in vitro and animal experiments, observing the effect of Eucheuma seaweed pigment glycoprotein (SPG) on the proliferation of mice hepatocarcinoma cells, the expression level of PCNA and Bcl-2 protein of tumor tissue, the inhibition rate and other indicators.Methods:1 In vitro experiment:Different concentrations of SPG (100、50、10mg /L) were cultured [...]
Objective To investigate the inhibition effects of SPG on mice hepatoma by giving different doses of SPG in H22 cell, and discussed the mechanism from caspase-3 and bax protein expression in hepatoma cells, proliferation of hepatoma cells, serum levels of TNF-a, tumor weight and body weight aspects.Methods Fifty mice were inoculated with hepatoma H22 cells [...]
Objective In our daily life, extensive deep burns, diabetes, trauma and other factors often damage the integrity of normal skin tissue structure and physiological function. How to effectively repair the wound, rebuild the skin tissue structure and restore the normal physiological function of the skin has now become a very difficult point of the experimental [...]
Nasopharyngeal carcinoma(NPC) is one of the commonest carcinomas with a high degree of malignant Phenotype in Southern China.especially those of Cantonese origin. Both the geographic pattern and familial aggregation of incidence are two main characteristics of NPC. The carcinogenesis of nasopharyngeal epithelium is a multi-stage, multi-path and multi-mechanism process, which is involved in the effects [...]
Objective:To investigate the effects and explore the related mechanisms of Inositol Hexaphosphate (IP6) on human liver cancer cell line HepG2 in vitro.Methods:HepG2 cells were cultured in vitro and treated with different concentrations of IP6 Its effects on the cells were evaluated by the follow experiments:1 MTT assay was used to evaluate the effect of IP6 [...]
Objective:To investigate the effects of high mobility group box protein 1 (HMGB1) on the proliferation, migration and apoptosis of human pulmonary artery smooth muscle cells (HPASMC) in vitro.Methods:We cultivated the HPASMC for 5th to 8th generations, and then began the following experiments. The HPASMC were devided into five different groups according to various concentrations of [...]
Objective:The aim of this study was to observe the impacts of different doses of arsenic trioxide for morphological characteristics on osteoblasts cultured in vitro, Detect the impacts of arsenic trioxide for proliferation and apoptosis of osteoblasts and for BMP-2 gene expression of osteoblasts; We investigate the mechanism of arsenic for growth and metabolism of osteoblasts [...]
Objective:To observe the effect of ginsenosides Rg1 (Rg1) on the proliferation and differentiation of human umbilical cord blood hemopoietic stem cells (hUCB-HSCs) and investigate the underlying mechanism of action.Methods:Density gradient centrifugation and magnetic cell sorting assay were used simultaneously to separate and purify the hUCB-HSCs, The cell differentiation antigens were detected by flow cytometry. hUCB-HSCs [...]
Vascular smooth muscle cell (VSMC) is the only cellular component of vascular middle layer. Under physiological conditions, vascular smooth muscle regulates angiotasis by contraction and relaxation, and smooth muscle cells release some regulatory factors to maintain normal functions of blood vessels. Abnormal proliferation of VSMC, resulting in VSMC migration from media to intima, is critical [...]
[Objective] Esophageal cancer is the eighth most common cancer and the sixth leading cause of cancer related deaths worldwide. The incidence of esophageal squamous cell carcinoma (ESCC) is more frequent in China. ESCC has a very poor prognosis, with an overall 5-year survival rate of about 25% after surgery. Yet the molecular mechanism during human [...]
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