[Objective]In order to offer the inspiration on deeply bone marrow CD34+cells replication research invitro,we investigated the proliferative effection of bone marrow CD34+cells by Bone marrowMesenchymal Stem Cells (BMMSCs).[Methods]êVarioMACS was used for sorting of bone marrow CD34+cells, and then the bone marrowCD34+cell purity was tested by FCM. áIsolating Marrow Mononuclear Cells from abortion fetalbone explored by [...]
Objective:Ischemic postconditioning (IPO) can reduce the reperfusion injury. The signal transductionprotective mechanism not only can prevent apoptosis, but also involves cell cycle regulation. Ourgroup has proven that the IPO can induce cardiomyocyte proliferation by the expression ofspecific marker5–bromodeoxyuridine (BrdU), phosph-Histone H3(H3P), and Aurora B. It isevident that the IPO not only can reduce apoptosis, but [...]
Objective:Some studies show that Crocin can inhibit the proliferation and induce the apoptosis of various of tumor cells.This study aims to investigate the effect of Crocin on malignant biological phenotype in human lung adenocarcinoma cell line of A549and its molecular mechanism.Method:Different doses of Crocin were added to the lung A549cell line to demonstrate the biological [...]
OBJECTIVE:The observation of four Chinese medicine (Astragalus, Epimedium, snow lotus and Yougui drink) on the proliferation of rat bone marrow mesenchymal stem cells (BMSCs) to chondrogenic differentiation.METHODS:This study is divided rat bMSCs separation, culture, subculture and proliferation; induced to differentiate into cartilage-like cells and proliferation by transforming growth factor-β1(TGF-β1) in vitro observation of Astragalus, Epimedium, [...]
Objectives: Previous studies have confirmed that the application of Low dose Xirradiation (LDI) could affect the Fracture healing process, however, the mechanism ofwhich remains unclear. The aim of this study was designed to investigate the mechanismsof LDI’s promotion to fracture healing.Methods:1. BMSCs were harvested from rat fresh bone marrow and exposed to a0Gy、25mGy、50mGy、75mGy、100mGy、1000mGy X-ray irradiation. [...]
ObjectivesCD95(Fas/Apo-1) triggers cell apotosis, cell proliferation, migration and cytokine production following engagement by CD95ligand or by agonistic anti-CD95antibodies. CD95-mediated apoptosis in RA synovium is defective. Our aim is to study intracellular CD95signaling on cell proliferation and pro-inflammatory response, and evaluate the role of PI3K/Akt pathways in this process. We try to reveal the molecular mechanisms [...]
Background:Colon cancer is a common malignant tumor, which seriously threat to human life andhealth. The traditional surgical operation and chemotherapy or radiotherapy methods havetheir limitations, we need to find new ways to treat colon cancer. Studies show that heatshock protein90(HSP90) is an important intracellular molecular chaperones, involved invarious protein folding, assembly and transportation.HSP90plays an important [...]
Objective To investigate the effects of Cinobufacini(Cino) on the proliferation ofhuman gastric carcinoma cell line BGC-823. To analysis the impact of cell cycle andapoptosis of human gastric carcinoma cell BGC-823treated by Cino combined withradiotherapy. To explore the potential mechanisms of Cino combined with radiotherapy.Methods1.Growth inhibition rate of Cino in gastric carcinoma cell BGC-823atdifferent concentrations(0μg/ml,0.113μg/ml,0.225μg/ml,0.45μg/ml,0.9μg/ml,1.8μg/ml)and different [...]
Objective:To compare the proliferation, migration, cloning and tumor-bearing among fourhuman gastric cancer cell lines respectively in vitro, and isolate, identify the sidepopulation(SP) cells from gastric cancer cell lines such as AGS, BGC-823, SGC-7901orMKN-28.Methods:Human gastric cancer cell lines, include AGS, BGC-823, SGC-7901and MKN-28,were cultured. The ability of proliferation was measured with cell growth curves andpopulation doubling [...]
Objective This study was to evaluate the effect of sorafenib alone or in combinationwith paclitaxel on human ovarian cancer cell SKOV-3.Methods MTT was used to examine the effects of sorafenib at differentconcentrations (1.5,3.0,6.0,12.0and24.0μmol/L) and different time points (24,48and72h) on proliferation of SKOV-3cells. Hoechst33258staining assay was used to observesorafenib-induced morphological changes; SKOV-3cells were treated with sorafenib [...]
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