First part: To investigate the expression of nuclear factor erythroid-2 related factor 2 (Nrf2)gene in chronic myeloid leukemia(CML)and explore its relationship with clinical characteristicand the correlation with thioredoxin reductase (TrxR). The expressions of Nrf2 and TrxR geneswere analyzed in 30 bone marrow prepares of CML patients in different phases, including 20 inchronic phase(CML-CP),3 in accelerated [...]
Objectives:To study the clinic value of bacteria detection using the polymerase chain reaction (PCR) in chronic rhinosinusitis by identifying aerobic bacterial content present on sinonasal mucosa specimens using PCR technique and comparing them with culture methods and to realize predominant aerobes in bacterial infection of CRS.Methods:Sinonasal mucosa specimens of 30 patients undergoing endoscopic sinus surgery [...]
Objective To determine the infection of Ureaplasma urealyticum(Uu) and Mycoplasma hominis(Mh) in urogenital tract of healthy prechoolers, and to discuss molecular epidemiology of Uu and Mh in this population group. Methods Urine specimens were collected from410preschoolers of the kindergarten in Qingdao, All specimens were frist be dectected by Urine routine examination, then discarded the unnomarmal [...]
BackgroundLeber hereditary optic neuropathy is a mitochondrial ophthalmopathy which is doing severe harm to youngsters, characterized clinically by acute or subacute impairment of binoculus central vision generally without eyeball pain or sore under press, revealing central scotoma and paracentral scotoma under examination of visual field. As manifested by epidemiological investigation, the attack rate of LHON [...]
Objective:Fluorescence in situ hybridization study of TEL/AML1 fusion and other abnormalities involving TEL and AML1 genes. Correlation with cytogenetic finding and prognostic value in children with acute lymphoblastic leukemia. According to different characters of TEL/AML1 positive signals classifying to study mechanism and clinical significance in the children suffering from acute lymphoblastic leuckamia. To study the [...]
Objective:To investigate the possible correlation between coronary heart disease and chronic periodontitis.Methods:Sub-gingival plaque samples and coronary atherosclemtic plaques were collected from 36 patients(13 Uygur cases,23 Han cases) who scheduled for coronary artery bypass surgery with periodontitis.50 sub-gingival plaque samples were collected from patients with chronic periodontitis. The DNA of bacteria was obtained from sub-gingival plaque [...]
Objective:To study the expression of folylpolyglutamate synthetase (FPGS) in methotrexate enantiomers resistant A549 cell lines (L-(+)-MTX和D-(-)–MTX)and the application of clinical specimens.Methods:A real-time fluorescence quantitative PCR method for detection of FPGS mRNA was established using SYBR Green I as fluorescence andβ-actin as reference. The method was evaluated by cycle threshold (Ct), correlation coefficient, slope, repeatability curve, [...]
Objective: Idiopathic pulmonary fibrosis (IPF) is an unexplained chronic inflammatory interstitial lung disease with characteristic pathological changes of usual interstitial pneumonia (UIP). IPF mainly presents diffuse alveolitis, structural disorder of alveolar units and pulmonary fibrosis, which is now considered a genetic or other aspects of patient susceptibility and the reaction against a particular disease of [...]
ObjectiveTo study clinical characteristic and imageology and histopathologic of MELAS syndrome, detect the common mutable point of Mitochondrial genome, which in order to diagnostic the disease as early as possible.Methods5 cases of MELAS syndrome were analyzed on clinical features, imageology, auxiliary examination, histopathologic of muscle. To extract the DNA in venous blood or muscle, The [...]
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