The treatment of intracellular bacteria is one of the clinical problems. Intracellular bacteria refers to the parasitic bacteria in the body’s phagocytes, Such as Mycobacterium tuberculosis, Salmonella typhi, Legionella pneumophila will cause chronic persistent infection. When these bacteria infect the human body, these bacteria will be engulfed by phagocytes. But phagocytes can not kill the [...]
Tumor is a disease which threatens seriously human health. At present the treatmenteffect of the traditional treatment method is not satisfactory. As a result of deep research oftumor, the tumor targeted drugs is becoming a trend of tumor treatment. The recombinanttargeted drugs generally consist of three parts: targeted carrier, effector molecule and linkerthat link the [...]
Ever since the identification of the first AIDS patient, researchers haven’t found the effective methods for prevention or treatment of HIV infection. DNA vaccine, especially the Prime-Boost strategy, is the most potential way among all drugs or vaccines, e.g. inactivated virus, subunit vaccine. It can induce the humoral and cellular immune response to prevent and [...]
Human serum album(HSA)is about60percent of the total protein of the human plasma. It is largelyresponsible for maintaining the normal osmolarity of the blood stream and also have other function such asanticoagulation、clean the free radicals et al. HSA is widely used in anti-shock, treatment of burns,bleeding caused acute hypovolemia by surgery or accidental. At Present，on sale [...]
To construct and high level express the human anti-bFGF ScFv and Diabody inPichia pastoris and study theirs biological functions.The gene of human anti-bFGFScFv was subcloned from plasmid pCOM3X-1A/ScFv, the variable light (VL) andheavy (VH) genes were respectively cloned from ScFv, The V genes were fused byoverlap PCR to produce Diabody fragments with a linker of [...]
6kD early secreted antigenic target, ESAT6 is a secreted protein presented in early culture filtrate of Mycobacterium tuberculosis with low molecular weight, which can induce intense cellular immunoreaction. It is only secreted by pathogenicity mycobacterium like Mycobacterium tuberculosis instead of nonpathogenicity ones, such as BCG, so it can be used in early diagnosis of tuberculosis [...]
6kD early secreted antigenic target, ESAT6 is a secreted protein presented in early culture filtrate of Mycobacterium tuberculosis with low molecular weight, which can induce intense cellular immunoreaction. It is only secreted by pathogenicity mycobacterium like Mycobacterium tuberculosis instead of nonpathogenicity ones, such as BCG, so it can be used in early diagnosis of tuberculosis [...]
6kD early secreted antigenic target, ESAT6 is a secreted protein presented in early culture filtrate of Mycobacterium tuberculosis with low molecular weight, which can induce intense cellular immunoreaction. It is only secreted by pathogenicity mycobacterium like Mycobacterium tuberculosis instead of nonpathogenicity ones, such as BCG, so it can be used in early diagnosis of tuberculosis [...]
Objective:To express the recombinant hZP3 peptides in Pichia pastoris yeast and E. coli Bacterium and to study the immunogenicity of the recombinant hZP3 peptides.Methods:The hZP3 DNA fragment was amplified by PCR and was inserted into an expression vector pGEB for the expression in E. coli. Then, the recombinant plasmid pGEBhZ3.2 were transformed to the E.coli [...]
Cucurbita maxima trypsin inhibitor I （CMTI-I） is one kind of the serine protease inhibitors from Cucurbita family which consists of more than 20 members. Because of their low molecular weights, simple structures and clear mechanism, CMTIs are used as tools for the study of the structure-function relationships of active proteins and their mechanism. Though natural [...]
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