Objective:NS is a protein that discovered and named in2002, which is very important and essential to the stem cell and tumor cell proliferation condition. This protein widely presents in the stem cells and tumor cells in the nucleus, and can play the biological effects of cells by combining with a protein named P53. The pathological [...]
Nucleostemin (NS) is a newly discovered protein in embryonic stem cells and adult central nervous system stem cells, located in the nucleolus. differentiated cells and mature tissue cells have not been found. NS contains a basic domain, its function related to the localization in nucleolar, as well as the interaction with the P53under physiological conditions. [...]
As people living standard rise, the interior decoration is frequent, cause indoor air pollution is more and more serious.Formaldehyde, Our indoor primary pollutants, which poison human’s health increasingly attracted people’s attention. June 15,2004, the world health organization (WHO) affiliate international cancer institute (IARC) according to “formaldehyde can cause human nasopharyngeal carcinoma” this conclusion, will be [...]
Objective:Nucleostemin (NS), which is involved in indefinite proliferation of stem cells and cancer cells, is an essential protein that discovered in 2002, and NS can also keeps cells in non-differentiated state. NS is a p53-binding protein that mainly existing in the karyon or nucleoli of stem cells and cancer cells, we can change the pathological [...]
Objective:Through the made of subcutaneous tumor-bearing nude mice model of prostate cancer and the comparison of treatment results, to further study the Cellular specificity of shRNA transcription driven by PSMAe/p to silence NS gene expression and the effect of gene silencing on the NS; to further explore the gene transfer capability of Tf-PEG-PEI in vivo.Method:Modify [...]
Objective To establish the method of RT-PCR, real-time PCR and nested RT-PCR to detect expression of Nucleostemin(NS) in various tumor cells and alimentary canal cancer , and to analyze the clinical significance for diagnosing alimentary canal cancer.Methods Tumor cell lines (SGC、A549、D95、SW480、U937、PC3) were cultured, cancer tissues, paraneoplastic tissue and the corresponding peripheral blood from patients of [...]
Objective: to discuss the mechanism and the function of NS by differentiating rat bone marrow stem cells into osteoblasts in vitro.Methods: The BMSCs isolated from the femur and tibia of 4~6-week-old male SD rat were cultured in vitro. The BMSCs of passage 3 were divided into two parts and cultured in different medium. Part A [...]
ObjectiveBased on selection of NS as “target gene”,use RNA interference techniques, by creating a non-viral vector system to transfer the interfere plasmid effectively and targetly into the prostate cancer cell in vivo and vitro, so interfere sequence can express specitly in the prostate cancer cell, silencing the specific cancer gene, which provided experimental and theoretical [...]
A novel tumor cell line, named F6, was acquired during the processes of inducing human embryonic bone marrow mesenchymal stem cells (MSCs) to differentiate into dendritic cells (DCs) by GM-CSF and IL-4 in vitro. The mechanism of tumorigenesis of F6 cells needs to be address .We used the methods as following:(1)Fluorescent differential display (FDD) was [...]
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