In order to prepare the highly specific and highly sensitive anti-myoglobin monoclonal antibodies whichare used for monitoring clinical cardiovascular and cerebrovascular diseases, two-step method was used in this study for the synthesis of human myoglobin total gene, and prokaryotic expression system was used to express myoglobin (Myo) protein which was purified through affinity purification subsequently. [...]
Object:Study on Clinical significance and correlation cTnI﹑MYO﹑CK-MB and Hemorheology in Patients with acute myocardial infarction。Methods:Control study between 80 cases of acute myocardial infarction ,65 cases of medical examinations and 48 AMI cases with at tack o f chest pain were determined at different time , Fluorescence enzyme immunoassay was used, by which the serum cTnI﹑MYO﹑CK﹣MB, [...]
ObjectiveTo explore the values of combined determination of cardiac troponin I (cTnI), myoglobin (MYO) and creatine kinase isoenzyme MB mass (CK-MB mass) as well as combined measurement of creatine kinase (CK), aspartate aminotransferase (AST), lactate dehydrogenase (LDH) and alpha-hydroxybutyrate dehydrogenase (α-HBDH) in the diagnosis of viral myocarditis (VMC), and to study early and specific markers [...]
Objective: At present, Percutaneous Coronary Intervention (PCI) has become an important treatment of coronary heart disease and plays an increasingly important role. However, interventional therapy would produce a certain degree of myocardial injury, thus affects the prognosis of patients. Therefore, predicting the extent of myocardial injury before interventional therapy is conductive to screening high-risk patients [...]
Effect of Penehyclidine Hydrochloride on Myocardium during Cardiopulmonary Bypass Heart Surgery【Objective】To observe the effect of penehyclidine hydrochloride on myocardium during cardiopulmonary bypass(CPB) heart surgery through cardioplegic solution.【Methods】20 patients,ASAⅡ～Ⅲ,cardiac functional gradingⅡ～Ⅲ,age 20～60y scheduled for mitral valve replacement(MVP or MVR) surgery under CPB were included in this study.They were randomized into two groups:penehyclidine hydrochloride group(P group) and [...]
Ischemia reperfusion injury is a common and severe complication in surgery, it happens when ischemic tissue and organ regain blood flow, but the functional impairment and structure injury is not relieved, it may even worse than before. The injury does not occur only in the Ischemia/Reperfusion local site, but also the organ far apart by [...]
BACKGROUND:Physical sports including physical exercise and military training not only cause the injury of the heart due to the increased demand of blood and oxygen but also induce skeletal muscle damage because of the more movement of the muscle. At present, most of the cardiac makers such as creatine kinase and myoglobin are lack of [...]
Study background and objctive: In forensic practice, the Postmortem interval(PMI )which has been being one of the focus and emphasis forensic study is very important .With the rapid development of the molecular biology and information technology, Forensic pathology research also gradually develop in the field of molecular biology information.Currently we has also achieved some encouraging [...]
In normal physiological conditions, reactive oxygen species (ROS) like hydrogen peroxide and hydroxyl radical could be generated in the course of biological metabolism. If a large amount of ROS exist in vivo or the antioxidant function of organism decreased, the oxidation system and antioxidation system would be out of balance and oxidative stress would be [...]
Backgroundst Objective:About 13-50% of rhabdomyolysis is complicated with AKI and myoglobin is the key element. Literatures about myoglobin toxicity mainly focus on oxidative stress, but the results diverse. The excact mechanisms of myoglobin induced kidney injury remains unclear. Apoptosis often occurs in animal models of AKI or cultured cells. Endoplasmic reticulum stress (ERS) has recently [...]
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