AIM In this research,we will co-culture T lymphocytes from normal humans with MSCs which were isolated from Immune thrombocytopenia patients’s bone marrow, Umbilical cord and healthy people’s bone marrow.3days later, we will monitor the cytokines IL-2, IFN-y, IL-4and IL-10to illustrate the diffence of immunodulatory of three kinds of MSCs.Methods:Human bone marrow-derived MSCs were isolated by [...]
ObjectiveRabbit bone marrow stromal cells (MSCs) transfected by bone morphogenetic protein-2(BMP-2) gene and transforming growth factor-β1(TGF-β1) gene mixed with calcium alginate to repair defects of articular cartilage of rabbit knee, then to observe the result of repairing and compare the repairation effect of single gene and double gene.MethodExtract bone marrow cells from a four-week-old New [...]
Objective:The objective was to fabricate a novel tissue inducible nerve conduits, and Functional evaluationMethods:The microspheres were prepared with chitosan that coated with ligustrazine, application of the controlled release method to detect the release effect of the chitosan microspheres/ligustrazine in vitro chitosan microspheres coating ligustrazine were mixed with collagen to fabricate the tissue inducible nerve conduits, [...]
Objective:The objective was to fabricate a novel tissue inducible nerve conduits, and Functional evaluationMethods:The microspheres were prepared with chitosan that coated with ligustrazine, application of the controlled release method to detect the release effect of the chitosan microspheres/ligustrazine in vitro chitosan microspheres coating ligustrazine were mixed with collagen to fabricate the tissue inducible nerve conduits, [...]
Background & ObjectiveMouse bone marrow-derived mesenchymal stem cells (MSCs) are adult stem cells that have the potential to undergo multi-lineage differentiation into multiple connective tissue cell types. They have a high capacity in self-renewal and proliferation. Under different conditions it can differentiate into mesoderm tissues, such as osteoblasts and cadiocytes, while it also can differentiate [...]
Background & ObjectiveMouse bone marrow-derived mesenchymal stem cells (MSCs) are adult stem cells that have the potential to undergo multi-lineage differentiation into multiple connective tissue cell types. They have a high capacity in self-renewal and proliferation. Under different conditions it can differentiate into mesoderm tissues, such as osteoblasts and cadiocytes, while it also can differentiate [...]
ObjectiveIn vivo and clinical found that the suitable mechanical loading can stimulate bone growth and reconstruction an application in the treatment of fracture repair, orthodontic movement and distraction osteogenesis. While numbers of in vitro studies also show that proper stress loading can promote bone formation protein and its receptor, collagen, expression of OPN, VEGF, inducing [...]
Objective:To study the further role of ChM-Ⅰin inducing BMSCs into chondrocytes, the expressing plasmid pcDNA3.1(+)/ChM-Ⅰwas constructed, and transfected into the MSCs.We make it expressed stably,and verify that it is upregulated in the MSCs,which could make preparation for studying the further role of ChM-Ⅰin inducing BMSCs into chondrocytes and constructing the tissue engineering .Methods:1.Extract the total [...]
ObjectiveIn vivo and clinical found that the suitable mechanical loading can stimulate bone growth and reconstruction an application in the treatment of fracture repair, orthodontic movement and distraction osteogenesis. While numbers of in vitro studies also show that proper stress loading can promote bone formation protein and its receptor, collagen, expression of OPN, VEGF, inducing [...]
Objective:To study the further role of ChM-Ⅰin inducing BMSCs into chondrocytes, the expressing plasmid pcDNA3.1(+)/ChM-Ⅰwas constructed, and transfected into the MSCs.We make it expressed stably,and verify that it is upregulated in the MSCs,which could make preparation for studying the further role of ChM-Ⅰin inducing BMSCs into chondrocytes and constructing the tissue engineering .Methods:1.Extract the total [...]
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