ObjectiveTo explore how to isolate and culture the rabbit peripheral blood mesenchymal stem cells (PBMSCs) under the conditions of the skin injury, and to observe their differentiation potential into the bone and cartilage cells. The effective method for the isolation and culture of rabbit peripheral blood mesenchymal stem cells will provide the experimental basis for [...]
Objective To study the expression of stromal cells-derived factor land CXC chemokine receptor 4 (SDF-1/CXCR4) in the acute infarct myocardium in rat after implanation of hypoxic preconditioning bone marrow mesenchymal stem cells (HPMSCs).Methods MSCs were isolated and purified for culture in vitro and serial passage by the attachment culture method. The morphological changes of the [...]
Objective:To compare the chondrogenic potential of rabbit bone marrow-derived mesenchymal stem cells (BMSCs) and adipose tissue-derived stem cells (ADSCs) in vitro.Methods:ADSCs were isolated from the subcutaneous adipose tissue which was from 6-month-old New Zealand white rabbits’abdomen by mechanical digestion and enzyme digestion, and then cultured and amplified in vitro. BMSCs were obtained from femurs of [...]
Objective:Detect and analyze the therapeutic effect of bone marrow mesenchymal stem cells which carry human vascular endothelial growth factor-165 (hVEGF-165) gene on hind limb ischemia, and provide new ideas for the clinical treatment of lower limb’s ischemia.Method:The experiment set up three groups, which were group BMSC carrying hVEGF-165 gene, group BMSC and blank control group, [...]
Objective:To investigate the reparative effect and probable mechanism of mesenchymal stem cells (MSCs) on immunization-induced injury of the ovaries in mice.Methods:6-8 week-old wild-type female mice bearing ICR background were divided randomly into 4 groups, each of 12 mice. Mice of group A and B were injected with Freund’s adjuvant and defined as control group, whereas [...]
Radiation-induced injury often occurs in nuclear explosions, nuclear leakage, nuclear terrorism and the clinical radiotherapy course. The tissues with rapid proliferating and metabolism cells, such as the hematopoietic tissue and intestinal tissue is the most sensitive to radiation. Abdominal tumor patients received a certain dose of radiation whose intestinal stem cells in the crypts and [...]
0bjectives To establish an induction system for HUCB-MSCs differentiating into dopaminergic neurons and exploit the potential therapeutic cell type for Parkinson’s disease.Methods HUCB-MSCs of passage 5 were induced to differentiate into dopaminergic neural like cells using three different methods respectively:the antioxidate chemical method,the neurotrophic factor method and the chemical combined with neurotrphic factor combining method.Morphological [...]
Objective:To investigate the influences of Zuogui Pill on the proliferation and differentiation of mesenchymal stem cells cultured in vitro.Methods:1.Whole marrow method was used to separate and culture rat mesenchymal stem cells,and then they were identified.2.The medicated serum concentration of Zuogui Pill（high,middle,low）was prepared,and MTT method was used to detect the effect of medicated serum on proliferation,it [...]
Part one: The expression of hHGF in the transfected MSCsObjective : To investigate the transfection efficiency of mesenchymal stem cells by adenovirus vector encoding GFP gene (Ad-GFP) and the expression of hHGF in MSCs infected with adenovirus vector encoding hHGF gene (Ad-hHGF).Methods: Rat,s bone marrow-derived mesenchymal stem cells (MSCs) were isolated using Percoll desity gradient [...]
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