Objective: To study JARID1B gene affecting the cell proliferation, apoptosis and histone modulation in HL-60 cells line, we design small interfering RNA(siRNA) targeting JARID1B gene.Methods:⑴Four siRNA segments targeting JARID1B gene were designed and transfected into HL-60 cells by LipofectamineTM2000. The optimal segment was screened by RT-PCR.⑵Cell growth affected by JARID1B siRNA was determined by MTT. […]

The self-renewal and directed differentiation of hematopoietic stem cell is regulated by a variety of hematopoietic growth factors, such as interleukin-1 (IL-1), interleukin-3 (IL-3), interleukin-5 (IL-5), granulocyte-macrophage colony stimulating factor(GM-CSF). IL-3 can stimulite the proliferation of pluripotent hematopoietic stem cells and induce the differentiation of myeloid stem cells and myeloid progenitor cells, and the mature […]

10. September 2013 · Comments Off · Categories: Oncology · Tags: , , , , ,

Objective:To investigate the biological effects of sCD40L on inleukemia HL-60 cells and its possible molecular mechanism in regulating the proliferation, differentiation, and apoptosis. And provide certain theoretical basis for rhsCD40L to be used in clinical practice.Methods:1) HL-60 cells were co-incubated with sCD40L of different concentrations. MTT assay were used to detect the cell proliferation and […]

10. September 2013 · Comments Off · Categories: TCM · Tags: , , ,

Background and objectives:Acute promyelocytic leukemia (APL) is characterized by abundant malignant promyelocyticblasts in the bone marrow and severe life threatening bleeding tendency. The genetic hallmark ofAPL is the balanced reciprocal translocation t (15; 17) (q22; q12), resulting in rearrangement ofthe retinoic acid receptor alpha gene (RARa) located onchromosome 17 and the promyelocyticgene (PML) on chromosome […]

31. August 2013 · Comments Off · Categories: Oncology · Tags: , , ,

As the blood system cancer, leukemia cells have the characteristics of cellular heterogeneity. The HL-60 cells, commonly used human myeloid leukemia cell line, have displayed the character of malignant phenotypes and shown the heterogeneity in cell morphology in cell cultures. What can be sure is, with the increase of emerging rate of tumor, various biological […]

Objective:A112 is a tamibarotene dimethylaminoethyl ester which is considered as the candidate compound for treatment of acute promyelocytic leukemia (APL) and other types of leukemia. Our goal in this study was to evaluate its efficacy of anti-cancer activity, beginning by studying its inhibitory effects on human promyelocytic leukemia cells HL-60 and then comparing it to […]

08. January 2013 · Comments Off · Categories: Oncology · Tags: , , , , ,

Objective:Minimal residual disease in leucamia patients are the root cause of resulting in patients with relapsed, because of immunotherapy is to activate the patients’ immune responses, can effectively remove minimal residual leukemia cells. As immune effected cells in the body’s innate immune, NK cells played an important role in immune surveillance, early in the process […]

Objective:To explore the reactive oxygen species (ROS) related mechanisms of xanthohumol (XN) bioactivity.Methods:The human promyelocytic leukemia cells HL-60 cell line was used as a study model. The cytotoxicity of xanthohumol on HL-60 cells were detected by Trypan Blue Test. Flow cytomitry techniques were used to detect apoptosis (cells were stained by Annexin-V FITC/PI), cell cycle […]

29. May 2012 · Comments Off · Categories: TCM · Tags: , , ,

Background and objectives:Acute promyelocytic leukemia (APL) is characterized by abundant malignant promyelocyticblasts in the bone marrow and severe life threatening bleeding tendency. The genetic hallmark ofAPL is the balanced reciprocal translocation t (15; 17) (q22; q12), resulting in rearrangement ofthe retinoic acid receptor alpha gene (RARa) located onchromosome 17 and the promyelocyticgene (PML) on chromosome […]

20. May 2012 · Comments Off · Categories: Oncology · Tags: , , , , ,

Objective:To investigate the biological effects of sCD40L on inleukemia HL-60 cells and its possible molecular mechanism in regulating the proliferation, differentiation, and apoptosis. And provide certain theoretical basis for rhsCD40L to be used in clinical practice.Methods:1) HL-60 cells were co-incubated with sCD40L of different concentrations. MTT assay were used to detect the cell proliferation and […]