Objective:Minimal residual disease in leucamia patients are the root cause of resulting in patients with relapsed, because of immunotherapy is to activate the patients’ immune responses, can effectively remove minimal residual leukemia cells. As immune effected cells in the body’s innate immune, NK cells played an important role in immune surveillance, early in the process [...]
Objective:To explore the reactive oxygen species (ROS) related mechanisms of xanthohumol (XN) bioactivity.Methods:The human promyelocytic leukemia cells HL-60 cell line was used as a study model. The cytotoxicity of xanthohumol on HL-60 cells were detected by Trypan Blue Test. Flow cytomitry techniques were used to detect apoptosis (cells were stained by Annexin-V FITC/PI), cell cycle [...]
Effects of Agaricus Blazei Murrill on the Proliferation, Apoptosis and NF-κB Activity of HL-60 Cells
Background and objectives:Acute promyelocytic leukemia (APL) is characterized by abundant malignant promyelocyticblasts in the bone marrow and severe life threatening bleeding tendency. The genetic hallmark ofAPL is the balanced reciprocal translocation t (15; 17) (q22; q12), resulting in rearrangement ofthe retinoic acid receptor alpha gene (RARa) located onchromosome 17 and the promyelocyticgene (PML) on chromosome [...]
Objective:To investigate the biological effects of sCD40L on inleukemia HL-60 cells and its possible molecular mechanism in regulating the proliferation, differentiation, and apoptosis. And provide certain theoretical basis for rhsCD40L to be used in clinical practice.Methods:1) HL-60 cells were co-incubated with sCD40L of different concentrations. MTT assay were used to detect the cell proliferation and [...]
Objective: To observe the effect of invasiveness and migration ability of leukemia cells treated by Glycyrrhetinic acid(GA),and study the mechanism.Method: The study was performed on cultured K562 and HL-60 leukemia cells, MTT assay was performed to examine the effects of glycyrrhetic acid on the proliferation of cells. The invasiveness of cells were tested by cell [...]
Leukemia is a malignant disease of the hematopoietic system. The risk of onset in children and young people is more than the other cancers. It is a significant research topic to seek natural and potent anti-cancer compounds to prevent and cure leukemia. Flavonoids are a large class of polyphenolic compounds, which are ubiquitously present in [...]
ObjectiveTo investigate the expression of CD147 on peripheral blood neutrophils from patients with psoriasis and healthy volunteers and the effects of CD147siRNA transfection on chemotaxis of neutrophils.To further identify the role of CD147 in the mechanism of psoriasis and the chemotaxis process of neutrophils.To investigate the feasibility of using gene therapy of siRNA technology to [...]
Objective To study the mechanism of mangiferin-induced apoptosis in HL-60 keukemic cells.Methods The standard HL-60 cells were divided into control and experimental groups,expression levels of apoptosis-related genes incluing bcl-2 and survivin were determined by RT-PCRResult 1. 20-250μumol/L mangiferin down-regulated the expression levels of bcl-2gene in HL-60 cells after treatment with mangiferin for 24 hours,and the [...]
Object: There is some article report that Vanadium-complex has anti-tumor activity. This paper studied tetravanadate VO3(molecular formula O13V4)’s affect to glycosyltransferase ppGalNAcT2′s activity, and weather the vanadium’s anti-tumor effect is related to ppGalNAcT2. Through add different concentration of metavanadata to the HL-60 cell and inhibit the expression of ppGalNAcT2, to observe the change of HL-60′s [...]
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