Objective: To design and select effective siRNA targeting human IGF-II and to construct thevector of recombinant RNA polymerase II hAFP/hTERT dual promoters-driven siRNAexpression against human IGF-II gene.Method:①According to the siRNA design principle, three siRNAs (siRNA1、siRNA2、siRNA3) targeting IGF-II mRNA and one negative control siRNA （NC siRNA）were designedand synthesized.25nM,50nM,100nM three different concentrations of siRNA1-3weretransfected into Huh7cells through [...]
Induced pluripotent stem cells (iPS cells) are derived from somatic cells that regainpluripotency by the nuclear programming with exogenous factors. iPS cells haveimmense potential applications in establishing disease models and understandingdisease mechanisms, cell therapies, etc. avoiding both immunologic and ethical barriersto their use. iPS–based disease models have made great achievements. However, it isnecessary to overcome [...]
Objective:To investigate the expression of ADM、EPOR in human primary hepatocellularcarcinoma and its relationship with the tumor pathological characteristics. And to detectthe relationship among the expression of ADM and EPOR with tumor size, tumormetastasis, pathologic types and other biocharacteristics of the mutual relations. Toinvestigate the role of ADM、EPOR in hepatocellular carcinoma angiogenesis and itspotential clinical applications [...]
ObjectiveTo analyze the diagnostic value of the combined detection of a new HCC marker,alpha-fetoprotein(AFP)-IgM immune complexes and liver function in hepatocellularcarcinoma (HCC).MethodsWe collected64cases of HCC and27cases of liver cirrhosis, and gathered venousblood5ml when patients were empty. Then we collected blood serum after centrifugation.We tested serum AFP-IgM complexes by ELISA and AFP by electrochemiluminescence.We collected patients’ [...]
Objective:To find the risk factors for postoperative pulmonary complications (PPCs) after hepatocellular carcinoma radical surgery, and to provide ideas for how to reduce or avoid the occurrence of postoperative pulmonary complications.Methods:collect the information of the patients with hepatocellular carcinoma whose hepatectomy were performed between January2008and December2009in the second affiliated hospital of Zhejiang university college of [...]
OBJECTIVES:This study was designed to explore the influence of hematoxylin on:①the inhibition ratio of hepatocellular carcinoma cell;②he adhesion of hepatocellular carcinoma cell Hep-G2;③the invasion of hepatocellular carcinoma cell Hep-G2;④he inhibition ratio of HUVEC;⑤. the angiogenesis of CAM.STUDY METHODS:Experimental part:①by using the MTT, this article tests the influence of hematoxylin with different concentrations on the inhibition [...]
Background and Objective: Hepatic focal nodular byperplasia(FNH) is the second most frequent benign liver tumor to hemangiomas in benign liver tumors.It reresents13.8%of all liver benign tumors and1.1%of all liver tumors. Hepatocellular Carcinoma is one kind of the most common malignant tumors worldwild,though it can be screening by Alpha fetal protein now,but30%of them is positive. Though [...]
Objective To detect the expressions of CD147, Connexin43(Cx43) and EGFR in the primary hepatocellular carcinoma (HCC) and explore the relationship between their expressions and the clinicopathological features of HCC. We were devoted to supply theoretical evidence for the gene targeted therapy of HCC.Methods Immunohistochemistry by PV6000detecting system was applied to detect the expressions of CD147, [...]
Objective:To analyze the relationship between the complications after resection in patients with hepatocellular carcinoma (HCC) and their clinical, operative and pathologic data, and to study the risk factors in the patients with the complications.Methods:Clinical and pathological data of 467 HCC patients from Jan.2002 to Dec. 2008 ware collected; and the risk factors in the patients [...]
Hepatocellular carcinoma is an important malignant tumor in China, the occurrence ofit is affacted by many factors, including viral and the genetic risk factors. In China, chronichepatitis B virus infection is the major risk factor for HCC. Recent studies have confirmedthat the occurrence of HCC is related with the chronic liver inflammation stimuli followedby HBV [...]
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