ObjectiveTo explore the effects of reduced glutathione (GSH) on the proliferation and activation of rat hepatic stellate cell-T6(HSC-T6) and signaling pathway of Nrf2/HO-1.Methods1. Choose a rat hepatic stellate cell strain (HSC-T6) as the research object. Rat HSC-T6were incubated with various concentration of LPS(1、0.1、0.01、0.001μg/ml). After24h,48h,72h of reaction, MTT colorimetric assay was employed to detect the Proliferation [...]

Objective:To investigate the antioxidative effects of L-carnitine on human ovarian granulose cells in vitro and in vivo and to explore its mechanism.Methods:The study was divided into two parts. The first part was to investigate the antioxidative effects and mechanism of L-carnitine on human ovarian granulose cells in vitro.Human ovarian granulosa cells were from40infertility patients (40cycles).20cases [...]

ObjectiveTo investigate whether apigenin(API) enhance apoptosis induced by TNF-related apoptosis-inducing ligand(TRAIL) through depletion of intracellular glutathione(GSH) in human hepatocellular carcinoma Hep G2 cell line.MethodsHuman hepatocellular carcinoma Hep G2 cell line and human embryo liver L-02 cell line were cultured in vitro. MTS assay was used to determine the cell viability. Flow cytometry (FCM) using propidium [...]

ObjectiveTo investigate whether apigenin(API) enhances apoptosis induced by TNF-related apoptosis-inducing ligand(TRAIL) through depletion of intracellular glutathione(GSH) in human gastric cancer BGC-823 cell line.MethodsHuman gastric cancer BGC-823 cell line and immortalized human gastric mucosa GES-1 cell line were cultured in vitro. MTS assay was used to determine the cell viability. Flow cytometry (FCM) after propidium iodide [...]

ObjectiveTo investigate whether apigenin(API) enhances apoptosis induced by TNF-related apoptosis-inducing ligand(TRAIL) through depletion of intracellular glutathione(GSH) in human gastric cancer BGC-823 cell line.MethodsHuman gastric cancer BGC-823 cell line and immortalized human gastric mucosa GES-1 cell line were cultured in vitro. MTS assay was used to determine the cell viability. Flow cytometry (FCM) after propidium iodide [...]

Drinking large quantities of alcohol and alcoholic beverages will lead to alcoholic liver damage (ALD) of the place, as China’s rapid economic development and improve people’s quality of life ever, the incidence of alcoholic liver disease have shown a rising trend. Phytochemicals on human health function is currently a hot topic in nutrition research, one [...]

The riboflavin play an important role in the chain of respiratory and organism metabolic as a flavin nucleotide.Lacking riboflavin may cause stomach troubles, and eyes, liver and nerve dysfunction, cause some dermatitis, versually impaired, fatigue and even unable to work, and even lead to bone abnormalities. Riboflavin is an integral parts of human metabolism. It [...]

ObjectiveTo investigate whether apigenin(API) enhance apoptosis induced by TNF-related apoptosis-inducing ligand(TRAIL) through depletion of intracellular glutathione(GSH) in human hepatocellular carcinoma Hep G2 cell line.MethodsHuman hepatocellular carcinoma Hep G2 cell line and human embryo liver L-02 cell line were cultured in vitro. MTS assay was used to determine the cell viability. Flow cytometry (FCM) using propidium [...]

[Objective] To explore acupuncture on climacteric syndrome effects of antioxidant stress, our research chose the ovariectomized SD female rats as the experimental animal models of climacteric syndrome. The experiments use of modern means of detection to detect the changes of reduced glutathione (GSH), oxidized glutathione (GSSG) and the changes of GSH/GSSG ratio in the uterus [...]

Objective1. To observe the effects of pretreatment with glutathione on the disturbance of glucose metabolism during the hepatic ischemia/reperfusion injury (HIRI) in rats.2. To investigate the possible mechanism of the disturbance of glucose metabolism during the hepatic ischemia/reperfusion injury (HIRI) in rats.Methods1. Experimental design: Seventy-eight healthy SD rats weighing 260~340 g were kept under constant [...]