Background and Objectives: Prostate cancer (Pca) is the most common malignancymale genitourinary system. In our country, the morbidity of prostate cancer was1.71per100,000cases in1993, and increased to2.0per100,000cases in1997, In2000it was up to4.55per100,000cases with increasing tendency year by year. The development and theprogression of prostate cancer have a variety of mechanisms which involved in a greatnumber of [...]
Objective Current treatments for bladder cancer include surgery, radiotherapy and chemotherapeutics. However, current treatments are not effective for recurrent and advanced disease. Gene therapy is one of the most important therapeutic straegy for improving the antitumor efficacy. Rdiosensitiveness which is the key point for radio therapy determines the antitumor efficacy.This study was designed to clarify [...]
Ischemic disease is always a hotpot research object in the region of Cardiovascular. Itis threatening the health of human being seriously. The current pharmacotherapy can onlyalleviate the contradiction between supply and demand on myocardial blood and it couldnot solve the problem of myocardial ischemia fundamentally. The mechanical myocardialrevascularization treatment, such as coronary artery bypass surgery, [...]
Objective To investigate pancreatic carcinoma and normal pancreatic tissuespecimen of PD-L1（programed de ath ligand-1） gene expression. To screen targetinghuman PD-L1mRNA siRNA effective sequence, and to construct RNA interferencelenti-virus targeting PD-L1. The reconstructed lenti-virus transfect human pancreaticcarcinoma cell line Patu8988, detecting the interfering efficiency. To lay the foundation forfurther investigation on the function and mechanism of [...]
ObjectiveTo study the effect of breast cancer associated fibroblast (CAFs) on breastcancer sells in vitro.MethodsThe CAFs and non-cancer associated fibrolasts (NAFs) were isolated fromprimary breast tumors and their paired normal breast tissue. The CAFs and NAFswere cultured in1640medium without the supplement of serum, and the supernatantwere collected.Breast cancer cell lines MCF-7and MDA-MB-468were cultured inthe collected [...]
Objective: To construct recombinant adenovirus vector carrying thehuman growth and differentiation factor-5 (GDF-5) gene and investigate thebiological effects of adenovirus mediated GDF-5 on extracellular matrixexpression in nucleus pulposus (NP) cells of human degenerativeintervertebral disc and explore a candidate method of gene therapy forintervertebral disc degeneration (IDD).Methods: (1) Recombinant adenovirus vector GDF-5 was constructedusing the AdEasy-1 [...]
Objective To construct human decorin (hDCN) prokaryotic expression vector PGEX-4T-1-DCN.Transform the recombinant plasmid into bifidobacterium of infantis, until fully expressed, take supernatant to observe the effect of bifidobacterium in K562cell proliferation, apoptosis and cell cycle.Method1.Use Double-Restriction enzyme method to amplify and recovery of DCN gene fragments, use molecular recombination technology to construction of prokaryotic expression [...]
Objective To evaluate therapeutic potential of fusion gene mFVⅡ/Fc for humanprostate carcinoma of severe combined immunodeficient (SCID) mouse model.Methods Human prostate carcinoma mice model was created by using Pc-3pros-tate cancer cells.When average volume of tumor was about100-150mm~3,eigh-teen SCID mice with human prostate carcinoma were randomly divided intotherapeutic group,negative control group and blank control group (n=6),separatelyreceiving [...]
Background:Breast cancer is one of the most common female malignant tumors. And more than1.2million women are diagnosed with breast cancer worldwide each year. Though breast cancer had once been considered largely as an illness of the Western word, it is a rising scourge in developing nations. In China, with the improvement of living standards, female [...]
Objective:Hepatic ischemia/reperfusion (I/R) injury is very important in transplant surgery. This study sought to investigate the potential of receptor activator for nuclear factor kappa B–fragment crystallizable (RANK-Fc) to protect against hepatic ischemia/reperfusion injury (HIRI) and clarify the possible mechanism for using this procedure.Methods:Ninety male BALB/c mice were randomly divided into 3 groups: a phosphate-buffered saline [...]
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