Backgroud and AimsRenal cell carcinoma (RCC) is a common malignant tumor in the urinary system, especially the recurrence and metastasis of postoperative is common to patients with advanced stage tumor, RCC is not sensitive to radiotherapy and chemotherapy,the anti-effect of immunotherapy is also insignificant.so the prognosis of patients is poor. Bioresearch of renal tumor cell [...]
ObjectiveOsteoarthritis, also known as degenerative arthritis, is due to old age or other reasons, such as congenital joint abnormalities, joint deformities, trauma and other causes of non-inflammatory articular cartilage degeneration and joint marginal osteophyte formation. It can produce clinical joint pain, limited mobility and joint deformities and other symptoms and severely affect the health of [...]
Objective:This project is to obtain the fusion protein molecules with activity of hIL-12 and HSP70 by constructing the eukaryotic expression vector of pIRES2-EGFP-hIL-12-HSP70, detecting its expression in 7402 liver cancer cells and observig its influence on the growth of 7402 liver cancer cell.Methods:hIL-12 gene was amplified from pBI121-hIL-12 plasmid by PCR and HSP70 gene was [...]
Objective:The generation of induced pluripotent stem cells (iPSCs) from somatic cells demonstrated that adult mammalian cells can be reprogrammed to a pluripotent state by the enforced expression of a few embryonic transcription factors. Human iPSCs raise the possibility of clinical application of autologous stem cell-based therapies without immune rejection or ethical concerns, and provide a [...]
Many factors and genes have been correlated with the occurrence and development of cancers. Inactivation of the tumor suppressor genes or activation of the tumor genes is a basic molecular event during carcinogenesis. Therefore, reactivation of tumor suppressor genes may represent an option of gene therapy for cancers. p16 has been known as a tumor [...]
Objective: To investigate the treatment efficiency of the double suicide gene therapy system on lung carcinoma and the security in vivo studies.Method: Use the method of reverse transcription PCR to confirm the expression of gene in SPC-A-1,SPC-A-1/TK+CD and SPC-A-1/pIRES-EGFP cells.The total of 30 mice were randomly divided into 6 groups then established the model of [...]
Key hinderances to gene therapy remain the lack of safe, efficiency, and targeting capability. Synthetic gene-delivery agents generally do not possess high transfection efficiency while showing advantages in low toxicity, low immune response, robust capability for various genes and cost effectiveness. Recent results showed the excellent efficiency for gene delivery of polycarboxybetaine ester (pCB-ester) when [...]
Part I The research on transformed Escherichia coli with IL-10 geneObjective:To successfully transform E.coli with murine IL-10 geneMethord:A murine IL-10 (mIL-10) gene fragment is inserted into the prokaryotic expression vector pET32a after the gene sequence and the plasmid were digested with endonucleases spot Ecoi I and HindⅢ, respectively. The sequence of recombinant plasmid pET32a/IL-10 was [...]
Objective:To clone OAS gene promoter and construct pGL3-Basic eukaryotic expression vector under OAS promoter,and then analysis its activity in L02 expressing the HCV core protein.Methods:①Adding restriction enzyme sites HindⅢand SacⅠ,OAS gene promoter region(-159～+82)according to the literature was amplified by polymerase chain reaction.②Right size band was gel received and digested by Sacl and Hindlll enzyme as [...]
Part one: The expression of hHGF in the transfected MSCsObjective : To investigate the transfection efficiency of mesenchymal stem cells by adenovirus vector encoding GFP gene (Ad-GFP) and the expression of hHGF in MSCs infected with adenovirus vector encoding hHGF gene (Ad-hHGF).Methods: Rat,s bone marrow-derived mesenchymal stem cells (MSCs) were isolated using Percoll desity gradient [...]
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