Objective： Disruptions of osteogenic pathway may prevent the progenitor cellsfrom undergoing terminal osteogenic differentiation, which leads to the developmentof osteosarcoma(OS). To induce OS cells to proceed osteogenic differentiation mayinhibits the malignant phenotype of OS cells. Therefore, We applied for AdEasyadenoviral vector system to construct recombinant adenoviral vector Ad-LMP-1andinvestigated its expression in osteosarcoma cells line U2OS [...]
Construction of Recombinant Adenoviral Vector Encoding LMP-1and Its Expression in Osteosarcoma Cells
Objective:To investigate the apoptosis-inducing and proliferation inhibition mechanism,which are induced by Diallyl Trisulfide (DT) or DT combined with cisplatin (DDP)on cisplatin-resistant human epithelial ovarian cancer cell lines SKOV-3/DDPconstructed in vitro.The results provide the experimental basis for clinical treatmentof cisplatin-resistant ovarian cancer.Methods:Cultured cisplatin-resistant human epithelial ovarian cancer SKOV-3/DDP cellswere randomly divided into the control group, DT-treated [...]
Objective:To investigate the Expression of ERCC1、BRCA1 and the correlation with platinum-based chemotherapy Clinical Efficacy in the advanced non-small cell lung cancer. Materials and Methods:We have retrospectively studied the 152 patients with advanced non-small cell lung cancer from January 2005 to May 2010 using platinum-based chemotherapy as first-line treatment. The tissue was examined by immuno-histochemical stains. [...]
Objective:To investigate the Expression of ERCC1、BRCA1 and the correlation with platinum-based chemotherapy Clinical Efficacy in the advanced non-small cell lung cancer. Materials and Methods:We have retrospectively studied the 152 patients with advanced non-small cell lung cancer from January 2005 to May 2010 using platinum-based chemotherapy as first-line treatment. The tissue was examined by immuno-histochemical stains. [...]
ObjectiveTo study whether hypoxia could induce the expression of HIF-2αin lung epithelial cells SPCA-1 compare the characteristics of induction of HIF-2αby hypoxia with HIF-1αand investigate the underlying mechanismsMethodsLung epithelial cells SPCA-1 cells were cultured in vitro under hypoxia or normoxia conditions, with different times, then we used RT-PCR and Western blot to determine the activation [...]
ObjectiveTo study whether hypoxia could induce the expression of HIF-2αin lung epithelial cells SPCA-1 compare the characteristics of induction of HIF-2αby hypoxia with HIF-1αand investigate the underlying mechanismsMethodsLung epithelial cells SPCA-1 cells were cultured in vitro under hypoxia or normoxia conditions, with different times, then we used RT-PCR and Western blot to determine the activation [...]
microRNA (miRNA) is a well-regarded research focus in the field of life sciences in recent years. It is a class of 21-25 nt conserved, short-sequences of single-stranded small RNA that has an important role in gene expression and regulation. microRNAs are found in humans and almost all organisms. Mature miRNA can make its target mRNA [...]
microRNA (miRNA) is a well-regarded research focus in the field of life sciences in recent years. It is a class of 21-25 nt conserved, short-sequences of single-stranded small RNA that has an important role in gene expression and regulation. microRNAs are found in humans and almost all organisms. Mature miRNA can make its target mRNA [...]
ObjectiveTo study whether hypoxia could induce the expression of HIF-2αin lung epithelial cells SPCA-1 compare the characteristics of induction of HIF-2αby hypoxia with HIF-1αand investigate the underlying mechanismsMethodsLung epithelial cells SPCA-1 cells were cultured in vitro under hypoxia or normoxia conditions, with different times, then we used RT-PCR and Western blot to determine the activation [...]
Vibrio parahaemolyticus (Vp) belonging to Vibrionaceae Vibrio, is a Gram-negativehalophilic bacteria, widely distributed in coastal areas, salt lakes, fish, shellfish and otherseafoods. It is an important pathogen causing food poisoning in coastal region.Consumption of seafoods that contaminated with Vibrio parahaemolyticus can causepatients with diarrhea, intestinal cramps, nausea, vomiting, fever, gastroenteritis typicalreaction, severe cases can cause [...]
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