Purpose To investigate the relationship between genetic polymorphisms in excision repair cross complementation group 1 ( ERCC1) 118 and ribonucleotide reductase M1(RRM1)37 and the response to gemcitabine/ cisplatin( GP regimen ) chemotherapy in advanced non-small cell lung cancer(NSCLC)..Materials and Methods Sixty patients with advanced NSCLC were analyzed. All the patients were treated with GP regimen [...]
Objective: To study the inhibitory effect of an PKM2 siRNA on the expression of CCND1 and its mechanism. Methods: Detect siM2PK-1′s influence on the proliferation and cycle by the colony formation assay and flow cytometry. Discover siM2PK-1′s effect on CCND1 via real-time quantitative PCR and Western blot. Verify the specification of siM2PK-1′s inhibition on CCND1 [...]
Objective:The analgestic effect of human prepropenkephalin(hPPE) was clear, which was an endogenous opioid peptides.Compared to the exogenous opioid substances,human preproenkephalin had fewer side effects. It provided material basis for analgesia study. The purpose of this study was to obtain human preproenkephalin gene sequence, construct human preproenkephalin gene recombinant vector pcNDA3.1(+)/hPPE through genetic engineering techniques,and express [...]
Background Alzheimer’s disease (AD) is an acquired intelligence drops syndrome with clinical manifestations as memory decline and other cognitive dysfunction. It is usually occult in onset, advances gradually and presents with diffuse encephalatrophy, especially in the temporal, parietal and prefrontal lobe. It also presents with marked dilation of the third ventricle and intralateroventricle and marked [...]
Background and objects:CHFR(checkpoint with FHA and ring finger) is a new cell cycle checkpoint gene,which is inactivated in human cancers. During stress mitosis,it has the function of delaying chromatin condensation and progression to the mitotic phase.Some research results show that, in many tumors,CHFR expression silencing because of the CpG island methylation. In tumor cells CHFR [...]
ObjectiveTo study the mechanisms of inhibiting proliferation of PHI , a kind of natural compounds, on multiple myeloma cell line U266 and illustrate effects of PHI on DNA methyltransferase;collect the data about PHI treated myeloma cells as a demethylation agent.Methodthe growth inhibition of U266 was observed by depicting cell growth curve and MTT ,the effect [...]
Objective:Mitochondrial DNA (mtDNA) is the only extranuclear genetic material; the genes coded by mtDNA participate in cell oxidative phosphorylation and energy metabolism. It is found that radiation can induce changes in expression levels of mitochondrial coded genes, but results of present studies are not in complete agreement. On the other hand, when accidental exposure or [...]
Objectives1. To research HepG2 cell infected with HBV and How HBV replicated and the expression of HBcAg.2. To constrret eukaryotes expression vector of HBV.,ore gene with its regulatory sequence.3. To research the expression of HBV core gene in HepG2 and the role of upper regulatory sequence.4. To research the integration mechanism of HBV DR area.Methods1. [...]
Objectives and Backgroud:To investigate the inhibition and mechanism of Temozolomide(TMZ) and PTX are very effective drug for malignant gliomas with significant survival increase.Preclinic and clinic investingations have demonstrated that the combination therapy of TMZ with PTX on glioma in vitro are empty. Our findings not only suggest the original TMT Paclitaxel in combination with great [...]
Objective (1) To examine the effects of extremely low frequency electromagnetic field (ELF) on cellular proliferation and apoptosis in hepatoma cell lines, which may indicate the role of ELF on the treatment of hepatic cell cancer (HCC). (2) To examine the effects of ELF on the expression of apoptotic genes in hepatoma cell lines, which [...]
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