Objective: To establish a high sensitivity, specificity, simple, rapid and low-cost method to detect rubella virus RNA.Methods: Primers were designed according to the Rubella’s selfish gene E1, and then the system of Reverse Transcription-loop-mediated isothermal amplification (RT-LAMP) to detect Rubella Virus RNA were established and optimized. Meanwhile, the specificity and sensitivity were evaluated. On the [...]
Objective: To establish a high sensitivity, specificity, simple, rapid and low-cost method to detect rubella virus RNA.Methods: Primers were designed according to the Rubella’s selfish gene E1, and then the system of Reverse Transcription-loop-mediated isothermal amplification (RT-LAMP) to detect Rubella Virus RNA were established and optimized. Meanwhile, the specificity and sensitivity were evaluated. On the [...]
ObjectiveTumor recurrence and metastasis are the leading causes of death in patients with cancer. Hematogeneous spread is one of the important ways of tumor metastasis. Detection of occult cancer cells in peripheral blood has recently received a great deal of attention regarding the monitoring cancer metastasis, and for novel strategies of adjuvant therapy. However, the [...]
ObjectiveTo study the diagnostic value of rSj26-Sj32 for the acute schistosomiasis japonica. And to investigate the value of amplification Sj26, Sj32 and Sj14-3-3 encoding gene by PCR or RT-PCR for diagnosis the patients with acute schistosomiasis japonica.MethodsThe rSj26-Sj32 were purified by Ni-NTA kits from BL21(pET28α-Sj26-Sj32), and were identified by SDS-PAGE and western-blot. To detect the [...]
ObjectiveDown’s syndrome,whose another name is trisomy 21,is the most common congenital chromosome aneuploidies and its incidence reaches to 0.13%at present. The incidence of DS remains in high level every year in China,which gives heavy burden to family and country.However,karyotype analysis applicated clinically can not be used in common laboratory,and also not be fit to large [...]
Objective:To detect the rearrangement of T cell receptor(TCR)gene for the diagnosis of mycosis fungoides(MF).Mycosis Fungoides is a low maliganent cutaneous T cell lymphoma,which has a variety of manifestation.It can be divided into 3 stages,including patche stage,plaque stage,and tumor stage.It can not be diagnosed easily by Histopathology until plaque stage.The presence of intraepidermal collections of atypical [...]
Objective1 .To detect the characteristics of clinical features in Shandong pedigrees with SCA.2. To make the molecular genetic diagnoses for patients and presymp-tomatic relatives of families in Shandong SCA.3. Screening the genetic, to make the diagnosis in patients with symptoms before.MethodsIn this study, all five family were found in Jinan Fourth People’s Hospital neurology clinic. [...]
Objective:To set up a new method detecting HSV DNA in serum of pregnant women,with high sensitivity,specificity and low-cost,and the clinical value of this technique were evaluated as well.Methods:We designed two specific LAMP(Loop-mediated isothermal amplification) primers for HSV-Ⅰand HSV-Ⅱrespectively,and established the reaction system to detect HSV by LAMP.To determine the best conditions of this method,we optimized [...]
Objective: To characterize the distributions and subtypes of the spinocerebellar ataxias (SCA)in Guangxi region.Methods: The SCAl、SCA2、SCA3/MJD、SCA6、SCA7 and SCA12 (CAG)n mutations were analyzed by polymerase chain reaction (PCR) and capillary electrophoresis(CE).Results: A total of 21 SCA patients and 19 presymptomatic individuals from 6 SCA families and their CAG repeat numbers expanded from 59～70 and 60～73 respectively. [...]
Objective Haemophilia A(HA) is the most common inherited bleeding disease, which is X-linked recessive disorders caused by mutations in some genes resulting in absence or deficiency of corresponding proteins.The factorⅧof Intron22 is the most common mutation Site.The factorⅧmutation can be detected in the 40%-50%Serious HA patients.It has been identified clearly that HA is a single-gene [...]
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