Research objectiveTo study the expression of Suivivin and P53gene in bladder adenocarcinoma, explore their roles and relationships in its occurrence and development,expect to get aids for diagnosis, treatment and predicting prognosis in patients with adenocarcinoma of the bladder.MethodsExpression of Survivin and P53protein were detected by immunohistochemical S-P method in40cases of bladder adenocarcinoma tissue,10normal bladder mucosa [...]
Objective: To investigate the relationship between intracranial atherosclerotic stenosisand genetics polymorphisms of rs1800787and rs1800790which are located in promoterarea in FGB gene.Method: The research was a case-control study. Patients in Department of Neurology ofNO.2People’s Hospital of Lianyungang were enrolled consistently from December,2010to February,2012. Basic characteristics,history and laboratory data including levelof plasma fibrinogen were recorded. And we [...]
【Objectives】To study the inhibitory effect of epigallocatechin-3-gallate(EGCG) on colorectal cancer cells and the mechanism of inhibition of colorectal cancer.【Method】Colorectal cancer cells (LoVo cells、SW480cells、HT29cells、HCT-8cells) were cultured and the proliferation ability of cultured cells in varying concentration of EGCG (10ug/ml、20ug/ml、35ug/ml)were tested by MTT analysis,and the cell apoptosis and cells cycle were detected with flow cytometry,and the gene [...]
Objective： To investigate the variation in the optineurin gene accociated with primaryopen-angle glaucoma (POAG) in chinese subjects.Method：100patients with POAG and60normal controls,aged50years old or more,wereenrolled in this study. DNA was isolated from peripheral blood of patients andnormal individuals. We used polymerase chain reaction(PCR) to amplify thecoding exons in OPTN gene. PCR products were be sequenced directly [...]
Objective To explore the relationship between rs9769826and rs9769506single nucleotide polymorphisms of INSIG1gene and type2diabetes in Han population of Qingdao, and to analyze the interactions between rs9769826mutation in INSIG1Gene and High total cholesterol(TC),waist-to-Hip Ratio (WHR), hypertension in type2diabetes mellitus.Methods Selecting125patients with type2diabetes and125normal controls by using frequency matched case-control study design. The rs9769826polymorphism of INSIG1gene was [...]
Objective:To investigate the association between obsessive-compulsive disorder(OCD) and rs518147polymorphism in the promoter of the serotonin2C receptor gene(5-HTR2C) in Chinese Han population.Methods:Direct sequencing technique was used to analyse the frequencies of rs518147polymorphism of5-HTR2C hemizygote (male) and genotype (female) in308patients (OCD:123male,185female) with OCD diagnosed according to Chinese classification of mental disorders (CCMD-3) or International Classification of Diseases-10(ICD-10) [...]
AIM：To investigate the changes of gene expressions of TLRs and theantioxidant capacities which occur in human umbilical cord mesenchymalstem cells （hUC-MSCs） during long-term culture in vitro, and to obtain theexperimental parameters and theory bases for the proliferation,differentiation,migration, damages repairing and immunoregulation of hUC-MSCs.METHODS：Human umbilical cords from neonates via caesarean sectionwere collected, hUC-MSCs were isolated and [...]
Background:Hemolytic disease of the newborn （HDN） is the hemolytic disease,due to the mismatchof red blood cell blood groups between mothers and fetus.The literatures reported that the red blood groupscausing HDN included the ABO,Rh,MNSs,Kell,Kidd,Yt,Duffy and Diego.However,the most commoncause is the mismatch of ABO and RhD between the mothers and fetus.The maternal IgG antibodies canthrough the placenta [...]
BackgroundThe neuroblastoma (neuroblastom NB),as the most common extracranial solid tumor, is one of the most common tumors in infants. It lists third in the tumors resulting in children’s death. NB originates from the primitive neural crest cells, may exist wherever the sympathetic nervous systems exist. Most primary tumors occur in the abdomen and sometimes get [...]
Objective: Comparative study the differences of distribution frequency between colorectal cancer patients specimens and healthy normal people specimens on three different genotypes TT, TG, GG and rs3809728AA, AT, TT，analysis the association between the two site gene polymorphisms and colon cancer susceptibility. To further explore the relationship between two sites different genotypes and colorectal tumor stage, [...]
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