Objective: Nowadays, the most attractive theory and method of therapy in coronary heart disease (CHD) is therapeutic angiogenesis, which is based on vessel forming of early embryonic development to stimulate the growth of new vessels in myocardial ischemia zone and collateral circulation, representing the new direction of therapy in CHD. However, factors, the receptors and [...]
AIM:To study the protective effect of cacitonin gene-related peptide (CGRP) on human umbilical vein endothelial cells (HUVECs) injury induced by H2O2 and observed whether caveolae involved in the protective effect.METHODS : 0.1% FBS Cell culture medium for 24h synchronized cells; pre-incubated cells with CGRP as factor of protecting cell and H2O2 induced for the cell [...]
Objective:The lack of a functional vascular supply has hampered the construction of large TEB and its clinical applications of poor recipient bed vascularity. Inadequate vascularization after implantation leads to nutrient limitations, which then results in cell death in the tissue-engineered constructs. In this study we set up a co-culture model of HUVECs and HBMSCs, and [...]
Part 1 The Plasma Pharmacokinetics Study of Tea Polyphenols Injection in RatsObjective: To develop a simple and specific reversed-phase HPLC-UV method for simultaneous determination of （-） Epigallocatechin-3-gallate （EGCG） and （-） Epicatechin-3-gallate （ECG） as the main antioxidant active ingredients of tea polyphenols （TP）, and thereby to study their plasma Pharmacokinetics （PK） in rats.Methods: EGCG and ECG [...]
Objective To study the expression of Cathepsin S (Cat S) in hepatocellular carcinoma (HCC),adjacent tissues of HCC and normal liver tissues.; to detect the relationship between expression of Cat S and its clinical pathologic features in HCC.Methods We randomly collected 63 cases of HCC tissue and adjacent tissues of HCC which were operated in the [...]
Objectives:To investigate the expressions and significance of activin、inhibin、p16 and p53 in the tissues of endometrial carcinoma(EC)、endometrial atypical hyperplasia (EAH)、normal endometrium(NE).Methods:The expression of activin、inhibin、p16 and p53 protein in tissues from 60 cases of endometrial carcinoma,30 cases of endometrial atypical hyperplasia and 30 cases of normal endometrium were detected by using immunohistochemistry S-P method and image analysis [...]
Background and Objective The goals of this study were to image oncogene c-erbB2 using 99mTc-radiolabelled antisense oligodeoxy-nucleotides(ASODN) to bind complementally target gene to diagnose breast cancer. The target of antisense oligonucleotide is amplification ribonucleic acid mRNA of mammary tumor protooncogene c-erbB2. 9 mTc-ladled ASODN was connected with epidermal growth factor(EGF) to enhance uptake percentage of [...]
Hypoxia-inducible factor 1(HIF-1) is a heterodimeric transcription factor. HIF-1 protein consists of two subunits, HIF-1αand HIF-1 β,both subunits belong to a family of basic helix-loop-helix transcription factors containing a PER-ARNT-SIM homology domain. HIF-1αis the subunit regulated by cellular O2 tension. HIF-1 regulates transcriptional activation of genes (more than 60), including erythropoietin, vascular endothelial growth factor, [...]
Background and objectsAlzheimer disease(AD) is a neurodegenerative disease which developswith age, characterized by evident learning and memory impairment anddementia.Recent research suggested that increase of oxygen free radicalsproduction and reduction of cleanability play an important role in cell apoptosisof AD.Oxygen free radicals are must produced in mitochondria and effect onmitochondria’s respiration fuction in turn.The abnormal structure [...]
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