Objective:To investigate the effect of ardipusilloside I on the proliferation and apoptosis of mucoepidermoid carcinoma Mc3 cells and explore its anti-tumor mechanism.Methods:The inhibitory effect of ardipusilloside I of Mc3 cells was measured by MTT assay. The apoptotic ratio and cell cycle analysis were determined by flow cytometry. Cell apoptosis was observed by fluorescence microscope techniques [...]
This paper presented a comprehensive summary and disscussion on the anticancer alkaloids obtained from plants and it highlighted the research on the alkaloids from Cassytha filiformis. This experiment investigated the methods to extract the alkaloids from Cassytha filiformis and addressed the priliminary analysis of its main chemical components. In addition,the experiment also examined its effects [...]
This paper presented a comprehensive summary and disscussion on the anticancer alkaloids obtained from plants and it highlighted the research on the alkaloids from Cassytha filiformis. This experiment investigated the methods to extract the alkaloids from Cassytha filiformis and addressed the priliminary analysis of its main chemical components. In addition,the experiment also examined its effects [...]
This paper presented a comprehensive summary and disscussion on the anticancer alkaloids obtained from plants and it highlighted the research on the alkaloids from Cassytha filiformis. This experiment investigated the methods to extract the alkaloids from Cassytha filiformis and addressed the priliminary analysis of its main chemical components. In addition,the experiment also examined its effects [...]
8Objective:1.To investigate the effect of MTX(included (+)MTX and (-)MTX)on the proliferation of ECV304 cells and to explore its mechanisms.2. A rapid and sensitive liquid chromatography-tandem mass spectrometry assay (LC-MS/MS) was developed and validated for the quantitative determination the concentration of methotrexate (MTX) enantiomers in the intracellular and extracellular fluid.Methods:1.ECV304, HepG2, A549 cells were cultured. The [...]
Objective: Previous studies suggest that polymorphonuclear neutrophils (PMNs) may be involved in triggering reperfusion-induced cardiomyocyte apoptosis through release of reactive oxidant substances, yet there is no evidence showing that activated PMNs could directly elicit apoptotic cell death due to the method limitation for differentiation of cell types in in vivo study. The aim of the [...]
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