ObjectiveTo explore how to isolate and culture the rabbit peripheral blood mesenchymal stem cells (PBMSCs) under the conditions of the skin injury, and to observe their differentiation potential into the bone and cartilage cells. The effective method for the isolation and culture of rabbit peripheral blood mesenchymal stem cells will provide the experimental basis for [...]
Survival and Differentiation of Autologous Bone Marrow Mesenchymal Stem Cells in Injured Spinal Cord
Objective:To observe survival and differentiation of autologous bone marrow mesenchymal stem cells(BMSCs) in semitransected spinal cord.Methods:36 male SD rats were randomly divided into BMSCs autograft group (ie, experimental group) and PBS control group, for each group of 18 rats.The rats of autologous BMSCs transplantation were intercepted on the left tibia and fibula in order to [...]
ObjectiveThe circumstance of skeletal muscle growth is complex, not only by the role of force in different ways, but also by nerve and various cytokines. Research at the cellular and molecular level will help to elucidate the important signaling pathway, which mechanical factors regulate the differentiation of myoblast, and is expected to reveal the mechanism [...]
Parkinsons disease (PD) is a neurological disorder that usually affects old people. The pathological basis of PD is the degeneration of the dopamine neurons which project from substantia nigra to the striatum, resulting in the reduction of dopamine in the brain and a series of clinical manifestations such as shaking, hypermyotonia and cinesipathy. Currently, medication [...]
Objective:To isolate and culture human umbilical cord mesenchymal stem cells (hUCMSCs) in vitro. To explore an ideal method to induce the differentiation from hUCMSCs into neuron-like cells and provide some evidence for the transplantation of hUCMSCs for spinal cord injure, different cytokines were used to induce the differentiation.Methods:The hUCMSCs were isolated from the human umbilical [...]
Objective:To observe the effect of ginsenosides Rg1 (Rg1) on the proliferation and differentiation of human umbilical cord blood hemopoietic stem cells (hUCB-HSCs) and investigate the underlying mechanism of action.Methods:Density gradient centrifugation and magnetic cell sorting assay were used simultaneously to separate and purify the hUCB-HSCs, The cell differentiation antigens were detected by flow cytometry. hUCB-HSCs [...]
Objective:To investigate whether there is keloid-derived mesenchymal stem cells in human keloid. Isolation, cultivation and identification of biological characteristics with human keloid-derived mesenchymal stem cslls(hKMSCs).Methods:The hKMSCs were isolated from human keliod with two-step enzymatic dissociation, selection by subculture. The surface antigen phenotype (CD29, CD34, CD44, CD45, CD90) and the cell cycle analysis was analyzed by [...]
Objective:To establish the methods of isolation and expansion of mesenchymal stem cells(MSCs) derived from human umbilical cord; To construct and purify protein keratinocyte growth factor 2(KGF-2) and TAT-KGF-2, then study their effects on proliferation of human umbilical cord MSCs(hUC-MSCs); To investigate the effect on adipogenic and osteogenic differentiation induced by KGF-2 and its mechanism.Methods:Tissue culture [...]
Objective To induce human bone marrow-derived mesenchymal stem cells (hBM-MSCs) to differentiate into cardiac phenotypic cells (Cps) by coculturing hBM-MSCs with neonatal Sprague-Dawley(SD) rat ventricular myocytes(NRVM) indirectly and detect miRNAs profile between hBM-MSCs and Cps. Then to find out functional miRNAs which can induce hBM-MSCs to differentiate into Cps and study molecular mechanisms of differentiation [...]
OBJECTIVE:Curculigo orchioides (Amaryllidaceac) has long been used in the clinical of traditional Chinese medicine for the treatment of osteoporosis. The main active phenolic compound isolated from Curculigo orchioides is Curculigoside (CCG). CCG may have antiosteoporotic effects, but the mechanisms underlying the effects remain unclear. In the present study, the effects of CCG on the proliferation, [...]
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