Objective:The aim of this investigation is to exam the the expression of PPARy2mRNA under the effect of glargine insulin, detemir insulin and human insulin in3T3-L1adipocytes and possible mechanisms.Methods:1. Culturing3T3-L1preadipocytes in vitro is to differentiate it into mature adipocytes. There were4major sample groups based on the different types of induction medium containing insulin. They are glargine [...]
Objectives: Previous studies have confirmed that the application of Low dose Xirradiation (LDI) could affect the Fracture healing process, however, the mechanism ofwhich remains unclear. The aim of this study was designed to investigate the mechanismsof LDI’s promotion to fracture healing.Methods:1. BMSCs were harvested from rat fresh bone marrow and exposed to a0Gy、25mGy、50mGy、75mGy、100mGy、1000mGy X-ray irradiation. [...]
Background and ObjectiveMesenchymal stem cells (MSCs) has strong self-renewal capacity and multi-differrentiation potential. As the seed cells, MSCs has broad application prospects in the field of cell therapy, tissue engineering and regenerative medicine. It can provide new treatment options for the major diseases of intractable diseases, such as peripheral nerve injury, spinal cord injury, bone [...]
0bjective To understand the feasibility and methods of red fluorescent protein(RFP)transfection in glioma stem cells by RFP-Lentivirus vector HIV-1．Methods The gliomastem cells were cultivated in serum free medium,then,immunostained with stem cellsurface marker antibodies-CD133, Nestin. The human brain glioma stem cells wastransfected with the lentivirus vector HIV-1containing red fluorescent protein(RFP)gene．Cells expression of RFP were observed by [...]
Objective: To research the basic biological characteristic of hUC-MSCs. To explorethe optimum condition to extract HHK and observe its biocompatibility withhUC-MSCs. Adopting different methods to induce hUC-MSCs differentiating toSCs,then to explore the role HHK has played on the process and find the optimummethod of them so as to provide theoretical grounds for the application of [...]
Rheb (Ras homolog enriched in brain) is a homolog of small G-protein Ras, andfunctions as a small GTPase. There are two types of Rheb in human body, GDP-Rheb andGTP-Rheb, and the later is active. Rheb regulates many progresses via mTOR such as cellsurvival, growth, proliferation, regeneration, energy metabolism, oxidative stress, and soon. In recently, it [...]
ObjectiveTo evaluate the feasibility of human fetal aorta-derived EPCs transfusion for thetreatment of atherosclerotic lesions, we isolated, cultured and identified endothelialprogenitor cells (EPCs) from human fetal aorta. The capability of EPCs differentiating intoendothelial cells was also analyzed. And then we evaluated the role of intravenouslytransfused human fetal aorta-derived EPCs on atherosclerotic lesions in apolipoprotein Eknockout(apoE-/-) [...]
Outside information are received, processing and perception by the visual system. The visual information is preliminary processing in the retina and then transmitted to the brain. Visual information transmission path is passed through the synapses between retina neurons. Retinal axons converging to the optic nerve, the way of the action potential of visual information is [...]
ObjectivesOval cell proliferation and profiled differentiation were involved in the formation of hepatocellular carcinoma (HCC) precancerous lesions. It was significant to take appropriate measures to block the HCC cell differentiation in cancerprevention and treatment. As the main effective continent of traditional Chinese medicine herb-Sophora flavescens. matrine has been used in the clinical treatment of liver [...]
Background:With the wide range of applications of mesenchymal stem cells (BMSCs) in clinical, BMSCs has become one of the hot research in seed cell. The mechanism of bone marrow mesenchymal stem cells differentiation in vitro is not clear, previous studies often focused on a number of bone morphogenetic proteins(BMP), and few of other genes, and [...]
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