Objective To study the clinical efficacy of Dendritic Cells (DC) combined Cytokine-induced Killer cells (CIK) adoptive cellular immunotherapy for malignant tumors.Methods Peripheral blood mononuclear cells(PBMC) were isolated from21patients with malignant tumors using Blood Cell Separator. DC were induced from the adherent cells and the CIK cells were induced form the suspension cells. DC and CIK [...]
Objective:To investigate the surface marker、CEA expression of DC and the antitumor efficiency of the special cytotoxic T lymphocytes (CTLs) activated by dendritic cells of colorectal cancer patients transfected by adeno-associated virus mediated CEA gene by flow cytometry,we can arrive the conclusion that this method can lift the surface marker、CEA expression of DC and the antitumor [...]
Objective: To establish a simple and reliable model of renal allograft transplantationin rats. Providing a more practical and high acute rejection rate model for experimentalstudies of renal transplantation and to observe whether CD4+CD25-T cells induced byrecipient-derived immature dendritic cells（imDC） transfected with IKK2dn gene andloaded with donor antigen can prolong the survival time of kidney transplantation [...]
Purpose: To establish a fast protocol for the generation of dendritic cells (DCs) fromhuman Peripheral Blood Mononuclear Cells (hPBMCs) in vitro. To construct HIV-1pseudovirus containing enhanced green fluorescent protein (EGFP) gene. To understand theinteraction between the virus and the cells.Method: High purity human CD14+monocytes were collected using density Ficollgradient centrifugation and MACS beads sorting system. [...]
Objective:To investigate the ovalbumin-induced asthmatic mice and the murine bonemarrow-derived dendritic cells(DCs) stimulated by the long-actingβ2agonist(salmeterol),and observe the inflammatory response of asthma and pro-inflammatory cytokine secretionof DCs.Methods:Asthmatic mice model was established via mice stimulation with ovalbumin (OVA).Fourty mice,6-8weeks of age, were randomly distributed into four groups: All mice weresensitized on days0and14by intraperitoneal injection of either [...]
Objection1. To approach rat orthotopic left lung allograft transplantation model based on the”three cuff technique”, which will provide a basic model for further research ofimmunological tolerance in post-lung transplantation.2. To explore the effective method of isolation,culture,and proliferation of Wistar ratbone-marrow derived dendritic cells(DCs) in vivo.And to observe the effect ofsTNFRI-IgGFc gene on the differentiation and [...]
Objective：To investigate The effect and The possible recent clinicalsignificance of The DC-CIK cell therapy on immune function of patients withhilar cholangiocarcinoma.Methods： Twenty patients diagnosed as HCCA after Da VinciRoboti-assisted Surgical Treatment in General Hospital of the SecondArtillery Corps of Chinese PLA from January2010to October2011weretreated by DC-CIK cells as experimental group，16patients at the same timewere treated [...]
BackgroudIn the clinical application of new antibiotics widely promoted, the new vaccines also got considerable development, the treatment of infectious diseases had achieved a great success. However, in the past a few decades, the prevalence of allergic diseases had grown obviously. Food allergy and related disease in the global range increased rapidly, about4%-8%of children and1%of [...]
[Objective] Establishing a practical, simple and efficient culture method to obtain high purity and large numbers of different mature state of dentritic cells(DC) from rat bone marrow. It also could control its mature phase to get different phenotypes of DC, such as imDC, smDC and mDC. Then, we analyzed its differences in mediating immune tolerance [...]
Tuberculosi（sTB）infected by Mycobacterium tuberculosis is a kind of infectiondesease. It has high infection rates and mortality. TB is serious harm to human health.Our country accounted for16%of new and relapse cases of TB that were notified in2010. Although these two years the number of TB cases declined, but the humanimmunodefici ency virus and M.tuberculosis infect in [...]
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