ObjectiveThis study was to observe the expression of vitronectin, the situation of cytoskeleton remodeling, cell migration and blood vessels forming in HUVEC trained by high glucose. And to survey the influence of vitronectin on cytoskeleton remodeling, cell migration and blood vessels forming in HUVEC.MethodsThe first part:HUVEC were cultured respectively by normal and high glucose. The [...]
Probiotics are widely used in food industry during the past two decades, They areidentified to have many good efforts to their host，although the mechanisms involvedare not fully understood. Most probiotics have a long history of safe use and fall intothe category of organisms classified as “generally regarded as safe”. Some reportsabout negative probiotic effects have [...]
Liquid crystal was the mesophase between liquid and crystal, it was the combination ofmobility and order in nature. Liquid crystal was the pervasive phase in living organisms, it wasalso one of the important structure modalities to keep our vital phenomena. Most of the mineralcrystals and collagen fibers are closely related in bone formation. Studying the [...]
Cinobufacini is a traditional Chinese anti-tumor drug and widely used in clinic experiences. But little is known about its effect on the cells. In this study, we used atmic force microscope (AFM), converted fluorescence microscope,CCK-8cell proliferation experiment, laser scanning confocal microscope (LSCM), flow cytometry (FCM) and transmission electron microscope (TEM) to detect the different tumor [...]
Objective: The cytoskeleton of eukaryotic cells is a network structure composed of many kinds of structural and contractile proteins and plays an important role in maintaining cell shape and contraction. There are three types of cytoskeleton: microfilaments of actin, microtubules and intermediate filaments 0f various molecules. The microfilaments are a complex, plastic, dynamic network structure, [...]
ObjectiveTo observe the effects of Fasudil Hydrochloride on the injury of HUVECs induced by AngiotensinⅡ（ AngⅡ）, which influence on the change of cytoskeleton and the expression of Rho associated kinase（ROCK） and Phospho-myosin light chain（P-MLC）, and explore the possible molecular mechanisms of Fasudil Hydrochloride in cytoskeleton of the injuried HUVECs.MethodsNeonatal umbilical cord were digested into the [...]
ObjectiveTo observe the effects of Fasudil Hydrochloride on the injury of HUVECs induced by AngiotensinⅡ（ AngⅡ）, which influence on the change of cytoskeleton and the expression of Rho associated kinase（ROCK） and Phospho-myosin light chain（P-MLC）, and explore the possible molecular mechanisms of Fasudil Hydrochloride in cytoskeleton of the injuried HUVECs.MethodsNeonatal umbilical cord were digested into the [...]
Objective:Our aim was to design a model system by which bone cell responses to mechanical load could be examined in vitro in a 3D environment. So we utilized Bose ElectroForce 3200 with BioDynamic orthopaedic applications made mechanical strains (0%-5%) on rat’s osteoblasts, which aim to investigate the effects of uniaxial cyclic mechanical strain on the [...]
Objective:Our aim was to design a model system by which bone cell responses to mechanical load could be examined in vitro in a 3D environment. So we utilized Bose ElectroForce 3200 with BioDynamic orthopaedic applications made mechanical strains (0%-5%) on rat’s osteoblasts, which aim to investigate the effects of uniaxial cyclic mechanical strain on the [...]
Previous studies have demonstrated that the human systems, including musculoskeletal system, cardiovascular system, immune system, nerve-endocrine system and self-generate physiological coherence system, were affected in the space microgravity. For example, the microgravity induces the occurrence of bone loss, amyotrophy, cardiovascular dysfunction, decreases in orthostatic tolerance and cognitive function. So what effect could be caused for [...]
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