Objective:To study proteome differences between domestic strain uropathogenic E.coli (UPEC) 132,model strain abroad UPEC J96 and non-uropathogenic E.coli K-12 MG1655 whose genomic sequencing has been completed.Methods:1.Identificated UPEC 132,UPEC J96 and E.coli K-12 MG1655 by Gram stain,test of biochemical reaction and papC PCR.2.Counted the bacteria number of three strains and described their growth curves with turbidity [...]
There is an increasing tendency in the incidence of SANFH(steroid-induced avascular necrosis of femoral head). However,The pathogenesis of SANFH has not been totally understood until now.Every function in the living cell depends on proteins, proteins play an important role in the occurrence of disease, development, and implementation of the vesting. Post-genomic era as a prelude [...]
Shigella spp. is a group of Gram-negative, non-spore forming, facultative pathogens. The bacteria cause a disease called shigellosis in humans, an infection of the large bowel characterized by abdominal cramps, diarrhea, and fever. There are four different species of Shigella based on the differences in O antigen and some biochemical reactions. Among them, the Shigella [...]
Part one Establishment and differential analysis of protein profiles of human gallbladder tissueObjective To establish and optimize a stable and standard proteomic technique platform and differential analysis those proteins from cholesterol gallstone patients and control ones, this study is designed to comparative analysis of the proteome of gallbladder tissue from two groups, to provide experiment [...]
Zou Yongxin has identified CUL4B gene caused XLMR, and then analyzed the expression pattern, localization of CULAB and its role on the cell proliferation and cell cycle regulation. CUL4B as a member of Cullin family, which is the significant moity of ubiquitin ligase enzymes E3, plays an important role in protein polyubiquitinated and degradative regulation. [...]
Background and objectives:Nasophryngeal carcinoma(NPC) is one of the common malignant tumors in China, especially in the southern part of the country. Radiotherapy is considered to be a prevalent treatment in present. However, some clinical patients present local recurrence and local residue after radiotherapy, one of the important reasons is due to the radiation resistance in [...]
Shigella is a genus of gram-negative bacilli that causes bacillary dysentery in humans by invading and replicating in epithelial cells lining the lower gut. Shigella infection spreads by the fecal-oral route. Because of the low infectious dose (10 to 100 organisms), person-to-person transmission probably is the most common. Worldwide there are approximately 164.7 million cases, [...]
Part I Establishenment of Two-dimensional Gel Electrophoresis forProteomics of Rat Spinal Nerve TissueObjective: To investigate the application of two-dimensional gel electrophoresis in normal rat spinal nerve proteome studying artificial somatic-autonomic reflex pathway.Methods: Protein of normal rat L4 ventral root was extracted by powerful solubility lysis solution(Urea 9M, CHAPS4%,Tris 40 mM, DTT 65mM, PMSF 2mM, EDTA [...]
Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is one of the membrane-binding cytokines that belongs to the family of tumor necrosis factor (TNF). TRAIL has been shown to be a potent apoptosis inducer in a wide variety of cancer cells in vitro and to limit tumor growth efficiently in vivo without damaging normal tissues dependent on [...]
Hello,My name is Jack,Ph.D. Supervisor, Welcome to My WebSite