Objective:To investigate the influences of temperature on SCF/c-kit proliferation pathway and cell cycle of spermatogonia through rat spermatogenic cells cultured in vitro at different temperatures, to provide basic research data for the mechanism of spermatogenic impairment due to body cavity temperature (37℃).Methods:The isolated spermatogenic cells from adult male SD rat using mechanical methods and enzymatic [...]
Objectives:This study is to provided experimental evidence of the molecular mechanisms of HSP70 act on pulmonary carcinoma by observing expression of exogenous HSP70 gene in human lung cancer A549 cells and its effect on the A549 cells growth.Methods:(1)Alkaline lysis method was used to extract plasmid DNA, including recombinant eukaryotic expression plasmid pIRES2-EGFP-HSP70 and control plasmid [...]
PART ONEObjective: To investigate the effect of hepatocyte cell adhesion molecule (hepaCAM) on proliferation of renal cell carcinoma and its mechanisms.Methods: The vector containing hepaCAM gene was transfeceted into 786-0 cells, the expression of hepaCAM and c-Myc in 786-0 cells were measured by RT-PCR and western blot; Cell proliferation was detected by cell counting; the [...]
Intrauterine methylmercury exposure could lead to offspring brain developmental damage and neuronal cell cycle alteration. Cyclin D1 and p21 might have important functions within this cell cycle regulation. In this study, rat experimental model was established of intrauterine methylmercury exposure. Offspring brain regions were determined cell cycle alterations at different time points and with different [...]
Objective: Observe the arsenic trioxide （As2O3） on human lung adenocarcinoma cell lineA-549cell growth, proliferation, cell cycle arrest, apoptosis and other aspects, and to explore its mechanism.Methods: Choose man lung cancer A-549 cell lines as specimen. Select logarithmic growth phase cells and set the negative control group. After the cells being effected by different concentrations of [...]
PTPRO, located on the cell membrane, is a typical receptor-type protein tyrosine phosphatases, involved in glomerular albumin permeability and axon growth and guidance. In many tumor cells, the expression of PTPRO is silenced by methylation of CpG islands in promoters and deletion in chromosome. These data have demonstrated the growth-suppressor characteristics of PTPRO that are [...]
Objective:to study the anti-tumor Effect and Mechanism of Cardiac glycosides (digitoxin,digoxin, cedilanid) Methods:The human tumor Cells and normal cell tumor cells proliferation influence of Cardiac glycosides was test by MTT assay; With different concentration of the cardiac glycosides on HepG2 cell,Using hoechst33258 fluorochrome, the change of morphologic was observed by fluorescent microscope; Adopt Na+/K+-ATPase;with the [...]
Objective:To investigate the effects and mechanisms of IP6 on cell cycle and related regulatory factors protein expression of human heptoma cell line HepG2. Explore the growth inhibition of human hepatoma cell line HepG2 mechanism for the anti-tumor effect of IP6 provide a theoretical basis for application development.Methods:Will group is divided into low dose group (1.0mmol/L), [...]
Objective:The morbility and mortality of malignant tumors have been showing a tendency to increase in recent years.Cancer has become the first cause of death in urban and rural areas in our country.Presently, surgery,chemotherapy,radiotherapy and biotherapy are the main therapeutic methods.The traditional chemotherapeutics are restricted to tumor patients because of their heavy side reactions and multidrug [...]
Section I The expression of Cdt1 and Geminin in Jurkat cellObjective To explore the expression of DNA replication licensing factor Cdt1 and the DNA replication inhibitor Geminin in leukemic cell in order to provid evidence for pathogenesy, diagnose, treatment and prognosis of leukemia.Methods Cultivate leukemic cell of Jurkat in vitro,exponential phase of growth Jurkat cell(research [...]
Hello,My name is Jack,Ph.D. Supervisor, Welcome to My WebSite