ObjectiveTo explore how to isolate and culture the rabbit peripheral blood mesenchymal stem cells (PBMSCs) under the conditions of the skin injury, and to observe their differentiation potential into the bone and cartilage cells. The effective method for the isolation and culture of rabbit peripheral blood mesenchymal stem cells will provide the experimental basis for [...]
1、ObjectiveBezoar ginseng capsule is according to the “Yiqi detoxification”of the basic principles, contains a certain percentage the Vitro cultivated calculus bovis and ginseng. The use of serum pharmacology method can be study the effect of the drug-containing serum on HepG2 about human hepatoma cell cycle and apoptosis status and to explore its mechanism.2、MethodsBy a certain [...]
Objective:To investigate whether there is keloid-derived mesenchymal stem cells in human keloid. Isolation, cultivation and identification of biological characteristics with human keloid-derived mesenchymal stem cslls(hKMSCs).Methods:The hKMSCs were isolated from human keliod with two-step enzymatic dissociation, selection by subculture. The surface antigen phenotype (CD29, CD34, CD44, CD45, CD90) and the cell cycle analysis was analyzed by [...]
Maternal-fetal fluid balance is critically important during pregnancy and amniotic fluid is essential for fetal growth and development. As a pregnant temporary organ, the placenta plays a key role in regulation of fluid transfer and nutrition metabolism during pregnancy.Aquaporins (AQPs) are specific water channels that allow the rapid transcellular movement of water in response to [...]
Objects:To investigate the effect of Effect ofα-ZAL on osteoblasts cultured in vitro. It studied the effect ofα-ZAL on the proliferation and differentiation and protein expression of osteoblast in rats,in order to approach the mechanism of action of isoflavones phytoestrogen in bone metabolism.The further objective is to develop medicine of preventing and curing osteoporosis.Methods:1 Osteoblasts were [...]
Objective:Submandibular gland with endocrine function can secrete a variety of biologically active substances,becase the thyroid and submandibular glands are from the same gastrointestinal pancreatic endoderm differentiation and formation,their structure and function may be closely related.Recent studies have reported that submandibular glands can secrete calcitonin (CT),which suggested submandibular gland secretion of calcitonin regulate calcium and phosphorus [...]
Objective:To conduct the primarily culture of rat adrenocortical cells, and to observe the characteristics of growth and secretion in cell curture in different phase which will help offer experiment evidence for searching the most suitable time of adrenal cell transplantation.Methods:Adrenal glands were removed from two week old Wistar rats under sterile conditions, then the cortex [...]
Background:Endothelial progenitor cells(EPCs),which are considered to include a hematopoietic stem cell population,and were shown to be incorporated into foci of neovascularization.Asahara and his colleague isolated cells from peripheral blood which express CD34,Flk-1,or AC133 antigen-positive in 1997 and identified them EPCs.This finding,that circulating EPCs may home to sites of neovascularization and differentiate into endothelial cells in [...]
Objective.To establish a method of culturing mesenchymal stem cells from bone marrow,and provide seed cells for tissue engineering of bone.Methods:The mononuclear cell fraction of mesenchymal stem cells was isolated,then MSCs were cultivated in serum-containing medium and serum-free medium,comparing the cells growth conditions in the two different medium.Identification of surface markers and cell cycle analysis of [...]
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