Objectives: Previous studies have confirmed that the application of Low dose Xirradiation (LDI) could affect the Fracture healing process, however, the mechanism ofwhich remains unclear. The aim of this study was designed to investigate the mechanismsof LDI’s promotion to fracture healing.Methods:1. BMSCs were harvested from rat fresh bone marrow and exposed to a0Gy、25mGy、50mGy、75mGy、100mGy、1000mGy X-ray irradiation. [...]
Objectives: To investigate the effect and mechanism of the role of basic fibroblastgrowth factor combined with bone mesenchymal stem cells in promoting healing ofβ-rayirradiated skin injury.Methods:1.3SD rats （age:6-8weeks old）, clean grade, were killed using cervical dislocationmethod. We obtained the femur and tibia bone marrow under sterile conditions, isolatedand cultured bone mesenchymal stem cells （BMSCs） by [...]
Objective:Confirm human bone marrow stem cell inducing differentiation to nervecell in vitro environment and discuss the clinical curative effect and safety ofautoallergic bone marrow stem cells treat cerebral stroke through subarachnoidcavity planting.Method:Our study contain two parts:First part: Separate and cultivate BMSCssuspension from fluid of autoallergic bone marrow of patients(patients havedone autoallergic BMSCs planting treatment),then send [...]
Part I Culture, identification, osteogenic and chondrogenicdifferentiation of human bone marrow mesenchymal stem cell in vitro.Objective:To establish and consummate a set of methods of isolating，culturing and identifyingmesenchymal stem cells (BMSCs) from bone marrow and to discuss the capacity of humanbone marrow mesenchymal stem cells osteogenic and chondrogenic differentiation in vitro.Methods:1. Culture of bone marrow mesenchymal [...]
Background:With the developing of cancer research, the tumor microenvironment has aroused widespread concern by governments and academia fields in recent years. People gradually realized that tumor is heterogeneity, and only a small part of cells which we called cancer stem cells (CSCs) are the source of tumor formation and development. Moreover, there was a close [...]
Objective：To study the effects of bone tissue from osteonecrosis of the femoralhead on proliferation and differentiation of the dog bone marrow mesenchymal stemcells in vitro culture．Methods：Developing a dog model of the femoral head osteonecrosis induced byliquid nitrogen freezing. BMSCs were isolated from dog ilium bone marrow bycombination of gradient centrifugation and adherent wall culture．Different bonetissue [...]
[Objective] Research for Bone marrow-derived bone Mesenchymal stem cells (BMSCs) under the action of osteoblast differentiation inducers directional for different states of cells (Including a multipotent, designed to stem cells or precursor cells, bone adult bone cells multiple stages). And each stage cells were transplanted to rabbit after fracture model,Then observe different developmental stages of [...]
Temporal control of bioactive signals in tissue engineering is greatly desired.In thisstudy,microspheres-based PHBHO(poly 3-hydroxybutyrate-co-3-hydroxyoctanoate) ofencapsulated bFGF(human basic fibroblast growth factor) were produced using three differentParticle Fabrication technique (electrospinning technique,solvent evaporationtechnique,ultrasonic emulsification technique). Protein probably denaturation and microspheresdiameter was average 10um by electrospinning method. microspheres adhere by solventevaporation method.microspheres-particle size distribution uniform, better dispersion arefabricated by [...]
ObjectiveTo repair defects of articular cartilage of the rabbits by BMSCs of the rabbit compound chitosan/collagen scaffold materials. The target is to provide the support for clinical treatment of articular cartilage injury diseases and provide the evidence for stem cells repairing cartilage defects.MethodsThe BMSCs of rabbits was cultured in vitro, purified, amplificated, and marked by [...]
ObjectiveTo repair defects of articular cartilage of the rabbits by BMSCs of the rabbit compound chitosan/collagen scaffold materials. The target is to provide the support for clinical treatment of articular cartilage injury diseases and provide the evidence for stem cells repairing cartilage defects.MethodsThe BMSCs of rabbits was cultured in vitro, purified, amplificated, and marked by [...]
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