Cardiac rhythm disorders such as sick sinus syndrome (SSS) or atrioventricular nodalblock (AVB) are a serious and life threatening condition, requiring implantation ofelectronic pacemakers in over300,000people annually in the US. While highly successful,these devices also have significant shortcomings. As a result, numerous laboratoriesworldwide are developing biological pacemakers as a replacement or complement toelectronic pacemaker.Various approaches [...]
Objective: Chronic myelogenous leukemia (CML) is a kind of acquired malignancy which is caused by clonal proliferation after mutation in multiple potential stem cells. The discovery and study of Ph chromosome and fusion gene of bcr/abl contribute a lot to the diagnosis and therapy of CML.The disease arises as a consequence of a rare mutational [...]
Objective: 5-Aza-CdR(5-aza-2′-deoxycytidin)is a methyltransferase inhibitor agent.Some study have recently shown that 5-Aza-CdR has strongly antitumor activity by inhibiting the proliferation and inducing cell apoptosis,it also can induce several tumor inhibitors expression in several human tumors.XAF1 is a new tumor suppressor,was found recently,low expression in tumors because of gene hypermethylation. The aim of this study is [...]
It has been known that amniotic fluid stem cells (AFS) are able to differentiate into three germ layers in vitro. Myocardial cells derived from mesoderm, so AFS have the ablity to differentiate into cardiomyocytes. However, so far the induction effictiency of rhythmic contraction cell cluster derived from AFS are very low. Pig is a main [...]
With the improvement of people’s living standard and changes of dietary structure in recent years, the incidence of ischemic heart disease has increased rapidly in China. It is a heart disease which can lead to myocardial necrosis or degeneration of the heart caused by an imbalance of the oxygen supply and the oxygen consumption. The [...]
Part1 Primary culture of beagle dogs’adipose-derived stem cells and differentiation induction into myoblastsObjective:To explore the method of culture of beagle dogs adipose- derived stem cells (ADSCs) and differentiation induction into myoblasts .Methods: Adipose tissues were obtained from beagle dogs, and were isolated by enzyme digestion and cultured into ADSCs . The expression of surface antigen [...]
Objective: To find an efficient method to induce rabbit bone marrow mesenchymal stem cells different towards smooth muscle cells in vitro, so as to establish a stabile cells source for further study.Methods: Rabbit bone marrow mesenchymal stem cells were isolated from bone marrow by the method of density gradient centrifuge. By adhering culture, a further [...]
Objectives(1)To observe the differential expression of E-cadherin between human keratinocyte cell line(HaCaT cells)and human epiderma squamous cell carcinoma cell line(A431 cells);(2)To investigate the possible mechanisms of the abnormal E-cadherin expression in A431 cells.Methods(1)HaCaT cells and A431 cells were cultured in vitro;(2)A431 cells were treated with 5-azacytidine(5-azaC)or/and Trichostatin A(TSA);(3)The expression levels of E-cadherin mRNA and protein [...]
ObjectiveTo observe the effects of 5-azaC on proliferation and inducible nitric oxide synthase expression in normal human keretinocytes,and then explore the relation between methylation state and iNOS expression.MethodsPrimary normal keratinocytes were cultured and treated with 1μM 5-azaC for 24,72 and 120h,and the control group was treated with PBS. The activation difference in cell growth was [...]
Myocardial infarction is the main cause of cardiac death.Patients who have the occurrence of myocardial infarction get acute and chronic necrosis on a large number of myocardial cells,the absolute number of myocardial cells will be reduced only by fibroblasts to fill to form scar tissue,resulting in partial myocardial contractility weakening to disorder,myocardial function decreasing,and heart [...]
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