Objective To explore the effects of cigarette smoke exposure on pulmonary artery pressure and expressional levels of eNOS and NO of pulmonary vascular in rats.Methods twenty-eight male SD rats were divided randomly into control group(C group), smoke exposure1month group(Slm group),smoke exposure2months groug(S2m group)and smoke exposure3months group(S3m group)with7rats in each group. Right ventricular systolic pressure (RVSP) [...]
Objective: This essay is intended to reveal the protective effect of adiponectin on human cardiacmyocytes cultured with high glucose, which will offer a new train of thought to the treatment ofdiabetic cardiomyopathy. In the experiment, human cardiac myocytes are cultured. The activityof cell multiplication, the cell apoptosis, and the changes of the expression of Bax/Bcl-2 [...]
PartⅠConstruction and function identification of recombinantlentivirus vector expressing rno-miR-145Objective To construct a lentivirus vector expressing microRNA (miRNA)rno- miR-145 and transfect the vector into VSMC.Methods The miR-145 shDNA double chain template sequence was synthesizedartificially and put this template sequence clone LV3 pGLV/H1/GFP+Puro-miRNALentivirus plasmid. 293FT cells were transfected. Lentivirus particles(virosome) wereharvested and concentrated, then infected primary cultured [...]
Objective: To observe the effect of PPAR gamma (peroxisome proliferator activated receptor )agonist pioglitazone pretreatment on myocardial ischemia reperfusion myocardial apoptosis andon the expression of GRP78 and Caspase-12 proteins in rats.so as to explore the the endoplasmicreticulun stress signaling pathways for pioglitazone in protection of m yocardium.Methods: male Wistar rats, the ordinary diet feeding. Weight [...]
ObjectiveObserve the different components of fine particulate matter (PM2.5) to rat cardiac hemodyna-mic effects and Myocardial tissue Ang II content. Further explore the might factors of the different components of PM2.5to rat cardiac function change.MethodsWistar rats randomly are divided into four groups;①Organic Extract Group,OG;②Water Soluble Extract Group, WG;③Mixing Extract Group,MG;④ontrol Group; every rat of each [...]
Objective To investigate the effect and mechanism of pretreatment with atorvastatin，pioglitazone，atorvastatin plus pioglitazone on myocardial ischemia reperfusion injury in ratsmodel.Methods 54 male wister rats were randomly divided into shame-operated group （n=6,normal saline 2ml/d）, I/R group （n=12, normal saline 2 ml/d）, atorvastatin group (n=12, atorvastatin 20mg kg-1d-1),pioglitazone group (n=12,pioglitazone 3mg kg-1d-1) and atorvastati+pioglitazone group (n=12, atorvastatin20mg [...]
BackgroundThe rise of cellular adoptive immunotherapy in the1880s gives the patients with tumor anew hope. Natural killer (NK) cells play a key role in the antitumor immune responses, plus thecapability of NK cells to kill tumor cells mainly depends on the synthesis of stimulatory andinhibitory signals on the cells surface, so NK cells become a [...]
Objective: To explore the capability and clinical value of Gemstone Spectral CT coronaryangiography in inspecting plaque in coronary artery.Methods: 56 patients who diagnosed coronary artery disease from Jun 2011 to November2011 in our hospital were divided into 2 groups according to clinical symptoms：stable anginapectoris group(SAP) and acute coronary syndrome group(ACS). All patients undergoneGemstone Spectral CT [...]
Objective To studay troponin additive to pulmonary embolism severityindex for the forecast value of severity in patients with actue pulmonary embolism.Methods we conducted a retrospecitive analysis of two hundred andforty-five patients admitted with PE confirmed by CT pulmonary angiogram orVentilation perfusion lung scanning in the NO 1 hosipital of shanxi medicaluniversity.According to the grouping of [...]
Objective To make emphysema models by active immunization and cigarette smoking usingguinea pigs, Then observe the expression of MMP-9、MMP-12、IP-10 and the destruction ofpulmonary tissue and myocardial tissue in each model，to explore the role of the MMP-9、MMP-12、IP-10 in the emphysema which induced by active immunization.Methods The 24 guinea pigs were randomly divided into three groups, involving [...]
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