Study on the Effect of Human Dermal-derived Multipotent Stem Cells on Wound Healing in Diabetic Nude Mouse

26. June 2012 · Comments Off · Categories: Endocrine · Tags: Cell differentiation, diabetes mellitus, epidermal cells, human dermal-derived multipotent stem cells, wound repair

Title	Study on the Effect of Human Dermal-derived Multipotent Stem Cells on Wound Healing in Diabetic Nude Mouse
Abstract	Objective The model of diabetic nude mouse was established in this study. BrdU-labelled human dermal-derived multipotent stem cells were engrafted into the wound of the nude mice by means of injection. After designing control group, the differences of two groups of wound healing nude mice were compared by statistical analysis. The distribution and differentiation of human dermal-derived multipotent stem cells were observed in order to evaluate the influence on wound healing in diabetic nudemouse.Methods (1)Establishment of the nude mouse model: the use of streptozocin(STZ) by intraperitoneal injection once; (2)Separation, culture and purification of human dermal-derived multipotent stem cells; (3)Calculating of the rate of wound healing: application of a transparent membrane and statistical analysis to the differences; (4)Histopathological observation of the wound tissue was completed by Hematoxylin-eosin (HE) staining; (5)The distribution and differentiation of human dermal-derived multipotent stem cells: the specimens around wound were immunohistochemically stained and cell differentiation was observed in serial section.Results (1)The successful construction of the wound model of diabetic nude mouse; (2) human dermal-derived multipotent stem cells were isolated, purified and cultured successfully; (3)According to statistical significance, human dermal-derived multipotent stem cells increased significantly the rate of wound healing in nude mouse; (4)Compared with control group, the newborn epidermis of experimental group was thicker, cell layers and the layer were more obvious. Basal cells were arranged of single-layered columnar structure, combined tightly and integrated into organizational structure. (5)BrdU positive cells with brown nuclei aggregated in the epidermis, dermis and hypodermia. Some positive cells with brown cytoplasm appeared in the expressed keratin simultaneously in serial sections.Conclusion BrdU-labelled human dermal-derived multipotent stem cells were injected into the wound of the nude mice. These cells have the potential for differentiating into epidermal cells under the microenvironment of diabetic wound. Human dermal-derived multipotent stem cells can improve wound healing rate and promote diabetic wound healing.
Category	Endocrine
Keywords	Cell differentiation, diabetes mellitus, epidermal cells, human dermal-derived multipotent stem cells, wound repair,
FileType	PDF
Pages	131
Price	US$48.00
Buy Now	Buy This Research Paper Now!
Download
Donation	Donated for this research subject
Contact	E-Mail:medpaper@hotmail.com TEL:1-888-786-998A
FAQ	How to get this paper's electronic documents? 1, Click the "Buy Now" button to complete the online payment 2, Download the paper's electronic document from the successful payment return page/Or the system will send this paper's electronic document to your E-Mail within 24 hours
Favorite	ADD TO FAVORITE
Version	zh-cn

Perhaps You will be interested in these papers

2013-03-12 The Clinical Study on Treatment of Diabetic Foots by Si Miao Yong an Tang Combined with External Application of Chinese Herbal Medicine
2013-03-12 Effects of Erxian Decoction Treating Diabetes(Diabetes with Blood Stasis Syndrome of Qi and Yin Deifciency of Two) Clinical Study
2013-02-28 The Correlation of Diabetes Mellitus Shao Yang Pattern with the Location of Blood Vessels Appearing on the Patients’ Sclera
2013-02-28 Analysis the Relation between Blood Coagulation, Immune Related Index and0Level Diabetes Foot in Traditional Chinese Medicine Syndrome Type
2013-01-09 Advanced Glycation End Products(AGEs)Inhibite the Expression and Function of Renal D1R in STZ Induced-diabetic Rats
2013-01-09 The Correlative Research of Diabetes and Tuberculosis
2013-01-08 A Study About the Risk Factors of Type2Diabetes Mellitus with Mild Cognitive Impairment
2013-01-07 Study the Cornea Changes of Diabetic Rabbit by Laser Confocal Microscopy
2013-01-05 The Effects on Myocardial Fibrosis and Cardiac Function of Diabetic Rats by Using Hedysarum Polybotys Saccharide Alone or Combining with Irbesartan
2013-01-05 The Correlation between Glycosylated Hemoglobin and the Severity of Coronary Artery Disease and Cardiac Function