| Title | Extraction of Triterpenoids from Ganoderma Lucidum and It’s Protective Effect on α-amanitin Induced Liver Injury |
| Abstract | Ganoderma Lucidum is a very famous medicinal fungus in Asia, mainly distributing in China, Korea, Japan and so on, as well as has been known by Chinese for over 2000 year. As it was showed in the research, triterpenoids is one of the chiefly important bioactive constituents of Ganoderma Lucidums, recently reported to elaborate various pharmaceutical effects including hepatoprotective activity, antitumor and inhibiting the release of anti-histamines et cetera. However most investigations of triterpenoids worldwide have concentrated mainly on isolation and purification, as the result of it,the toxic effects of fractions obtained at every step of isolation and purification have not been ascertained.In the investigation, G.lucidum fruit bodies were extracted with 95% (v/v) ethanol boiling in an immersion heater after being pulverized. The ethanolic extracts were concentrated to dryness, redissoved in hot water and extracted with chloroform. The chloroform layer was extracted with saturated NaHCO3 solution and the extract was acidified to pH 2-3 with 6 mol/1 HC1 at 0℃. Crude extracts were purified to obtain different constituents by using TLC, silica gel olumn chromatography and HPLC.On the other hand, in order to track the constituents isolated at every phase, the amatoxin poisoning group, the several chiefly important bioactive constituents of Ganoderma Lucidum treatment groups, physiological saline treatment group were compared, determine ALT and AST activity in serum, as well as SOD, CAT and MDA activity in liver tissue, while observing the ultra structure of liver cell. Then it shows that most of crude extracts could reduce the destroy of amatoxin- induced injury in mice, while a bit of the extracts did not have the obviously protective effects. After administration of amatoxin in mice, the ALT,AST and MDA activity were rising obviously, compared with the normal group. However, in the chiefly important bioactive constituents of Ganoderma Lucidum treatment groups, the ALT,AST and MDA activity showed a download, especially the high dosage triterpenoids treatment group (200mg/kg) approached the normal group, followed by the water extracts of Ganoderma Lucidum treatment group(Fr.W) and the ethanol extracts of Ganoderma Lucidum (Fr.E).After administration of amatoxin in mice, the SOD and CAT activity came down, compared with the normal group, while the SOD and CAT activity showed a rise in the chiefly important bioactive constituents of Ganoderma Lucidum treatment groups, particularly the high dosage triterpenoids treatment group (200mg/kg) was the most close to the normal group, followed by the water extracts of Ganoderma Lucidum treatment group(Fr.W) and the ethanol extracts of Ganoderma Lucidum (Fr.E).Ultra structure of liver cells in mice of the normal group, a-amatoxin poisoning group, triterpenoids treatment group was compared. The results showed that numerous large vacuoles are very often observed in the a-amatoxin poisoning group, as well as the damage of organelles, comparing the normal group. However, after administration of triterpenoids, the liver cells revived, at the same time, the ultra structure was close to the lever of the normal group. It was indicated that triterpenoids had the significantly protective effects on amatoxin-induced liver injury in mice. |
| Category | TCM |
| Keywords | Ganoderma lucidum, Isolation, Liver injury, purification, Triterpenoids, α-amatoxin, |
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| Pages | 153 |
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