Objective:Constructing the fusion expression vector of CD59 gene and CD2 gene, which was steady expressed, is aim to research the reciprocity of CD59 and CD2 in T cell signal transduction and investigate CD59 transmitting signals to T cell.Methods:CD59 and CD2 genes were cloned by RT-PCR from the total RNA of Jurkat cell. CD59-T and CD2-T [...]
Objectives:To construct recombinant vector pSUPER-siCD55 pSUPER-siCD59 with siRNA technique. Detect the changes of T cell signal after transfected with pSUPER-siCD55 or pSUPER-siCD59, and investigate the synergistic effects of glycosylphosphatidyl inositol (GPI)-anchored protein CD59, CD55 in lipid raft-mediated transduction.Methods:recombinant vector pSUPER-siCD55 pSUPER-siCD59 was constructed and subsequently identified by PCR, restrictiion endonuclease reaction and gene-sequencing methods. Jurkat [...]
Objective(1) Analysis of the 26 TRBV CDR3 length and polymorphism in the peripheral blood of mother and son donors by Capillary Electrophoresis.(2) Analysis of the composition and character of the part of TRBV Functional gene CDR3 and TRBV Pseudogene CDR3 in the healthy donors samples by Cloning and Sequencing.(3) Analysis of the composition and character [...]
Objective:The aim of this study was to find the change of spectral patterns in CD4+ and CD8+T cell receptors(TCR) b-chains complementarity determining region 3 (CDR3) before and after immunized with HBV Vaccine. The peripheral blood was obtained from 20 donors (Buyi, Dong, Miao and Han). And to analyse the association between CD8+ T cell CDR3 [...]
Objective:1. To detect the CDR3 of TCR (3 chain gene repertoire in the peripheral blood mononuclear cells of Hemorrhagic fever with renal syndrome-vaccine in CD4+T and CD8+T cells after three Vaccination. Analysis of CDR3 molecular features of single peaks.2. Preliminary Discussion of the Relationship Between CDR3 molecular features and HLA Serotyping.3. Analysis of CDR3 molecular [...]
Objective:To investigate the efficiency of pVAX1-SA、pVAX1-GC and pVAXl-SG immunized in New Zealand rabbits by different routes.We further hope to explore the best effective route using in rabbit.Methods:The present study includes two parts:Test one:Identification and preparation of recombinant plasmids pVAXl-SA. pVAXl-GC and pVAX1-SG and pVAXl plasmid.First procedure in which extract a small sample of recombinant plasmids [...]
Objective:Investigated the method and ability of transdifferentiation between osteoblasts and adipocytes, thus to prepared for the further research of the regulation by gap junction communication.Methods:Took adipose tissue from groin of 3 months New Zealand white rabbit, isolated and cultured the adipocytes. Using inverted ceiling adherent culture method to dedifferentiate the adipocytes, and the 3rd generation [...]
Objective:To compare the chondrogenic potential of rabbit bone marrow-derived mesenchymal stem cells (BMSCs) and adipose tissue-derived stem cells (ADSCs) in vitro.Methods:ADSCs were isolated from the subcutaneous adipose tissue which was from 6-month-old New Zealand white rabbits’abdomen by mechanical digestion and enzyme digestion, and then cultured and amplified in vitro. BMSCs were obtained from femurs of [...]
Objective:To observe the rabbit skin graft rejection and the change of IL-2,IFNγ,GCs,T cell apoptosis in peripheral blood after the injection of recombination human interleukin-10, to explore the mechanism of recombination human interleukin-10 in transplantation of the rabbits skin..Methods:(1)36 rabbits were divided into two groups which were receptors and donors, each donor rabbit preserve skin for [...]
Objective:Detect and analyze the therapeutic effect of bone marrow mesenchymal stem cells which carry human vascular endothelial growth factor-165 (hVEGF-165) gene on hind limb ischemia, and provide new ideas for the clinical treatment of lower limb’s ischemia.Method:The experiment set up three groups, which were group BMSC carrying hVEGF-165 gene, group BMSC and blank control group, [...]
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