Objective To explore the effects of cigarette smoke exposure on pulmonary artery pressure and expressional levels of eNOS and NO of pulmonary vascular in rats.Methods twenty-eight male SD rats were divided randomly into control group(C group), smoke exposure1month group(Slm group),smoke exposure2months groug(S2m group)and smoke exposure3months group(S3m group)with7rats in each group. Right ventricular systolic pressure (RVSP) [...]
Objective: This essay is intended to reveal the protective effect of adiponectin on human cardiacmyocytes cultured with high glucose, which will offer a new train of thought to the treatment ofdiabetic cardiomyopathy. In the experiment, human cardiac myocytes are cultured. The activityof cell multiplication, the cell apoptosis, and the changes of the expression of Bax/Bcl-2 [...]
PartⅠConstruction and function identification of recombinantlentivirus vector expressing rno-miR-145Objective To construct a lentivirus vector expressing microRNA (miRNA)rno- miR-145 and transfect the vector into VSMC.Methods The miR-145 shDNA double chain template sequence was synthesizedartificially and put this template sequence clone LV3 pGLV/H1/GFP+Puro-miRNALentivirus plasmid. 293FT cells were transfected. Lentivirus particles(virosome) wereharvested and concentrated, then infected primary cultured [...]
Background And Objective:NS5ATP9is a trans-activator gene of HCV NS5A protein, screened by suppression subtractive hybridization (SSH). The present study shows that NS5ATP9gene is associated with a variety of tumor tissue. It involved in DNA repair, cell signaling pathways and the regulation of cell proliferation. But the reports about NS5ATP9expression in human tissues and cell lines [...]
[Objective]In order to offer the inspiration on deeply bone marrow CD34+cells replication research invitro,we investigated the proliferative effection of bone marrow CD34+cells by Bone marrowMesenchymal Stem Cells (BMMSCs).[Methods]êVarioMACS was used for sorting of bone marrow CD34+cells, and then the bone marrowCD34+cell purity was tested by FCM. áIsolating Marrow Mononuclear Cells from abortion fetalbone explored by [...]
ObjectiveIt is generally believed that the heart becomes a postmitotic and differentiated organ,atwhich point the cardiomyocytes withdraw from the cell cycle.Recenly,scientists have provedthat myocardial cells could reenter into the cell cycle on the condition of physiology andpathology.The cell signal mechanism of ischemic post-conditioning(IPO)to ischemicreperfusion injury,not only inhibits cell apoptotic,but slso regulates the cell cycle.The IPOprotect [...]
ObjectiveStudies have shown that smoking can cause the occurrence and development of chronicinflammation of pulmonary, which may be related to the imbalance of toxic substances on theimmune system. To explore the roles of antigen presenting cells in the lung of chronicinflammation, by investigating the effects of smoking on the expression of B7-1/B7-2and itsassociated ligands in [...]
Objective:Ischemic postconditioning (IPO) can reduce the reperfusion injury. The signal transductionprotective mechanism not only can prevent apoptosis, but also involves cell cycle regulation. Ourgroup has proven that the IPO can induce cardiomyocyte proliferation by the expression ofspecific marker5–bromodeoxyuridine (BrdU), phosph-Histone H3(H3P), and Aurora B. It isevident that the IPO not only can reduce apoptosis, but [...]
Objective:To investigate the effects of adiponectin on oxidative stress and apoptosis in Retinal pigment epithelium cultured with high glucose.Methods:The cells were divided into three groups:the normal glucose group, high glucose group and high glucose plus adiponectin group. After culture for24h,48h,72h Oxidative stress was evaluated by detecting levels of MDA and SOD in the supernatant of [...]
MRSA (methicillin-resistant Staphylococcus aureus), is a common pathogens to cause pyogenic infection and nosocomial infection. Because of the bacterium evolution and drug abuse, the resistance of bacteria has increased gradually, especially MRSA. It has become a global focus. MRSA has the characteristic of antimicrobial drug resistance, even began to resist to vancomycin which is considered [...]
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