Tissue engineering, an emerging multidisciplinary field to revolutionize the ways we improve the health and quality of life for people, promises the regrowth of adult tissue structure through the application of engineered cells and synthetic material. The scaffold used in tissue engineering provides the necessary support for cells to proliferate and maintain their differentiated function, and its architecture defines the ultimate shape of the new tissues. hybridization among biodegradable polymers, i.e., gelatin and chitosan with the bioactive inorganic species, i.e., Si-OH group and Ca²⁺ ions, may construct a promising scaffold for tissue engineering.In this work, gentamicin-sulfate （GS）-loaded gelatin-siloxane microshperes were synthesized and then integrated with gelatin-siloxane through a sol-gel processing. The mechanism of this sol-gel process was further explored. Moreover, gelatin-siloxane nano-fibers were fabricated by electrospinning. The main results are as follows:1. GS-loaded gelatin-siloxane microshperes were synthesized and then integrated with gelatin-siloxane hybrid scaffolds were through a combined processing of sol-gel, post-gelation soaking, and freeze-drying, and the as-synthesized scaffolds are antibacterial and biocompatible.2. The mechanism of the sol-gel process was investigated by determining of viscosity of the sols. It was indicated that a higher gelatin and GPSM concentration might lead to a higher reaction rate.3. When the viscosity of the sol, which is composed of 20% gelatin in 98% formic acid and appropriate GPSM, reached to 68224 mPa·s, the ultra-sleek gelatin-siloxane nano-fibers could be fabricated by electrospinning this sol at a 25 kV voltage, 2μL/min flow rate, and 30 cm air gap.4. Gelatin-siloxane nano-fibers fabricated by electrospinning showed in vitro bioactivity as they could biomimetically deposit apatite after soaking in simulated body fluid （SBF solution） for 7 days. 5. The rabbit bone mesenchymal stem cells （BMSCs） could adhere and spread on the surface of gelatin-siloxane nano-fibers. Moreover, BMSCs proliferated at a steady rate in the culture period and excrete lots of ECMs. Therefore, the gelatin-siloxane nano-fibers were biocompatible.