To improve the corrosion resistance of magnesium alloy (AZ91D), we present a novel approach to fabricate anticorrosive multilayer onto AZ91D substrates. Corrosion resistance properties of antcorrosive multilayer onto AZ91D were investigated by hydrogen evolution, Electrochemical impedance spectroscopy (EIS) and Potentiodynamic polarization (TAFEL) measurements. Cytocompatibility of antcorrosive multilayer onto AZ91D were investigated by MTT assay and alkaline phosphatase (ALP). The main work and conclusions were as follows:Multilayer were deposited onto magnesium alloy substrates via spin-assisted layer-by-layer (LbL) technique to form a (PEI/PSS/8HQ/PSS)25 multilayer onto magnesium alloy substrates.The multilayered structure was stabilized with glutaraldehyde (GA) as crossing linker, which was confirmed by Fourier transform infrared spectroscopy (FT-IR). The surface morphologies, elemental compositions of the formed anticorrosive multilayers were investigated by scanning electron microscopy (SEM), optic microscopy and energy dispersive spectroscopy (EDS), respectively. The degradation performance of the coated AZ91D substrates was investigated by hydrogen evolution. Electrochemical impedance spectroscopy (EIS) and Potentiodynamic polarization(TAFEL) measurements suggested that the deposited anticorrosive multilayers greatly improved the corrosion resistance properties of magnesium alloy substrates.Multilayer were deposited onto magnesium alloy substrates via spin-assisted layer-by-layer (LbL) technique to form a (PEI/GEL/Ce/GEL)25 multilayer onto magnesium alloy substrates. The anticorrosive multilayer was cross-linked by 5 % glutaraldhyde. The assembly process of cerium multilayer on magnesium alloy surface was monitored by contact angle. The result shows that the Uniform film are formed. Anticorrosive resistance of ceium multilayered magnesium alloy was investigated in 0.14 mol/L NaCl sultution via Electrochemical impedance spectroscopy (EIS) and Potentiodynamic polarization measurement (TAFEL), respectively. The result show that cerium multilayer can improve the corrosive resistance of magnesium alloy.The primary SD rats osteoblasts were cultured from the calvaria of new-born rats by the tissue piece culture method. The osteoblasts were purified by the difference of the adhibition time with other type of cells. It was identified by the morphology and calcium nodules staining. The third transfer of the cultured osteoblasts was used in the mechanical experiment.Conditioned mediums were prepared by immersion multilayer onto magnesium in the DMEM medium for 24 h. The conditioned mediums were centrifuged and supernatant was collected for further study. Cytocompatible of 8-HQ multilayered magnesium alloy was also studed according to MTT assay and alkaline phosphatase (ALP) measurement. In vitro study revealed that the deposited multilayers were cytocompatible.Conditioned mediums were prepared by immersion multilayer onto magnesium in the DMEM medium for 24 h. The conditioned mediums were centrifuged and supernatant was collected for further study. Cytocompatible of cerium multilayered magnesium alloy was also studed according to MTT assay and alkaline phosphatase (ALP) measurement. In vitro study revealed that the deposited multilayers were cytocompatible.